Synergic phototoxic effect of visible light or Gallium-Arsenide laser in the presence of different photo-sensitizers on Porphyromonas gingivalis and Fusobacterium nucleatum

Ghanbari, H. and Mousavi, S. A. and Forouzanfar, A. and Zakeri, M. and Shafaee, H. and Shahnaseri, S. (2015) Synergic phototoxic effect of visible light or Gallium-Arsenide laser in the presence of different photo-sensitizers on Porphyromonas gingivalis and Fusobacterium nucleatum. Dental Research Journal, 12 (4). pp. 323-330.

[img] Text
Synergic phototoxic effect of visible light or Gallium.pdf

Download (1MB)

Abstract

Background: According to the development of resistant strains of pathogenic bacteria following treatment with antimicrobial chemotherapeutic agents, alternative approaches such as lethal photosensitization are being used. The aim of this study was to evaluate the effect of visible light and laser beam radiation in conjugation with three different photosensitizers on the survival of two main periodontopathogenic bacteria including Porphyromonas gingivalis and Fusobacterium nucleatum in different exposure periods. Materials and Methods: In this in vitro prospective study, strains of P. gingivalis and F. nucleatum were exposed to visible light at wavelengths of 440 nm and diode laser light, Gallium-Arsenide, at wavelength of 830 nm in the presence of a photosensitizer (erythrosine, curcuma, or hydrogen peroxide). They were exposed 1-5 min to each light. Each experiment was repeated 3 times for each strain of bacteria. Data were analyzed by two-ways ANOVA and least significant difference post-hoc tests. P < 0.05 was considered as significant. After 4 days the colonies were counted. Results: Viability of P. gingivalis was reduced 10 and 20 subsequent to exposure to visible light and diode laser, respectively. The values were 65 and 75 for F. nucleatum in a period of 5-min, respectively. Exposure to visible light or laser beam in conjugation with the photosensitizers suspension caused significant reduction in the number of P. gingivalis in duration of 5-min, suggesting a synergic phototoxic effect. However, the survival rate of F. nucleatum following the exposure to laser with hydrogen peroxide, erythrosine and rhizome of Curcuma longa (curcumin) after 5-min was 10, 20 and 90 respectively. Conclusion: Within the limitations of this study, the synergic phototoxic effect of visible light in combination with each of the photosensitizers on P. gingivalis and F. nucleatum. However, the synergic phototoxic effect of laser exposure and hydrogen peroxide and curcumin as photosensitizers on F. nucleatum was not shown.

Item Type: Article
Additional Information: Cited By :6 Export Date: 16 February 2020 Correspondence Address: Mousavi, S.A.; Departments of Endodontics, School of Dentistry, Isfahan University of Medical SciencesIran
Uncontrolled Keywords: Laser therapy pathogenic bacteria periodontitis phototherapy
Subjects: WN Radiology . Diagnostic Imaging
WU Dentistry. Oral surgery
Divisions: Mashhad University of Medical Sciences
Depositing User: mr lib5 lib5
Date Deposited: 12 May 2020 09:25
Last Modified: 12 May 2020 09:25
URI: http://eprints.mums.ac.ir/id/eprint/18849

Actions (login required)

View Item View Item