Evaluation of CD4+ CD25+ FoxP3+ regulatory t cells and FoxP3 and CTLA-4 gene expression in patients wwith newly diagnosed tuberculosis in northeast of Iran

Ghazalsofala, R. and Rezaee, S. A. and Rafatpanah, H. and Vakili, R. and Ghazvini, K. and Heidarnejad, F. and Sobhani, S. and Valizadeh, N. and Azami, M. and Rahimzadegan, M. and Asnaashari, A. (2015) Evaluation of CD4+ CD25+ FoxP3+ regulatory t cells and FoxP3 and CTLA-4 gene expression in patients wwith newly diagnosed tuberculosis in northeast of Iran. Jundishapur Journal of Microbiology, 8 (4).

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Abstract

Background: Tuberculosis (TB) is the world's second most common infectious disease after Human Immunodeficiency Virus Infection/Acquired Immunodeficiency Syndrome (HIV/AID) and the most frequent cause of mortality especially in developing countries. T regulatory (Treg) cells, which have suppressive activity and express forkhead winged-helix family transcriptional repressor p3 (FoxP3), suppress the immune responses against pathogens such as Mycobacterium tuberculosis. There are controversial results regarding the role of FoxP3 expressing cells in the blood of patients with TB. Objectives: The aim of this study was to evaluate the frequency CD4+ CD25+ Treg cells, and FoxP3 and Cytotoxic T Lymphocyte Antigen 4 (CTLA-4) gene expressions in peripheral blood of patients with tuberculosis and patients with positive tuberculin skin test before and after Peripheral Blood Mononuclear Cells (PBMCs) activation with Purified Protein Derivative (PPD). Patients and Methods: In this cross-sectional study, Peripheral Mononuclear Cells (PBMCs) were isolated from peripheral blood of 29 patients with newly diagnosed pulmonary TB and 19 patients with positive tuberculin skin test. The PBMCs were activated with PPD for 72 hours. Activated cells were harvested, RNA was extracted and cDNA was synthesized. A real-time Taqman method was designed and optimized for evaluation of Foxp3 gene expression and SYBR Green method was used and optimized for evaluation of CTLA-4 gene expression. A flow cytometry analysis was used to evaluate the frequency of CD4+ CD25+ Foxp3+ regulatory T cells in both groups. Results: There was no significant difference in the frequency of CD4+ CD25+ FoxP3+ regulatory T cells between the two groups. Expression of FoxP3 and CTLA-4 in peripheral blood of patients with newly diagnosed TB was significantly lower than the control group after and before activation with PPD. Conclusions: The expression of FoxP3 and CTLA-4 in PBMCs of patients with newly diagnosed TB was low, which might suggest that Treg cells may be sequestered in the lungs. © 2015, Ahvaz Jundishapur University of Medical Sciences.

Item Type: Article
Additional Information: Cited By :7 Export Date: 16 February 2020 Correspondence Address: Asnaashari, A.; Chronic Obstructive Pulmonary Disease Research Center, Mashhad University of Medical SciencesIran
Uncontrolled Keywords: Cytotoxic T-lymphocyte associated antigen 4 (CTLA-4 Antigen) Regulatory T-Lymphocytes Tuberculosis CD4 antigen complementary DNA cytotoxic T lymphocyte antigen 4 interleukin 2 receptor alpha messenger RNA transcription factor FOXP3 tuberculin adaptive immunity adolescent adult aged Article CD4+ CD25+ T lymphocyte cellular immunity clinical article controlled study cross-sectional study CTLA gene DNA determination female flow cytometry FoxP3 gene gene expression human human cell leukocyte activation lung tuberculosis lymphocyte count lymphocyte function male peripheral blood mononuclear cell protein function regulatory T lymphocyte RNA analysis tuberculin test Human immunodeficiency virus Mycobacterium tuberculosis
Subjects: WC Communicable Diseases
Divisions: Mashhad University of Medical Sciences
Depositing User: mr lib5 lib5
Date Deposited: 05 May 2020 09:08
Last Modified: 05 May 2020 09:08
URI: http://eprints.mums.ac.ir/id/eprint/18857

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