An identified antioxidant peptide obtained from ostrich (Struthio camelus) egg white protein hydrolysate shows wound healing properties

Homayouni-Tabrizi, M. and Asoodeh, A. and Abbaszadegan, M. R. and Shahrokhabadi, K. and Nakhaie Moghaddam, M. (2015) An identified antioxidant peptide obtained from ostrich (Struthio camelus) egg white protein hydrolysate shows wound healing properties. Pharmaceutical Biology, 53 (8). pp. 1155-1162.

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Context: Ostrich (Struthio camelus) egg possesses a high amount of food proteins and thus plays an important role in nutrition. Objective: Ostrich egg white proteins were hydrolyzed with pepsin and pancreatin to examine its antioxidant properties and further characterized the most active peptide. Materials and methods: Ostrich egg white protein hydrolysate (OEWPH) was fractionized using reversed phase high-pressure liquid chromatography (HPLC). The antioxidant activity of OEWPH and its HPLC fraction were investigated based on their scavenging capacity1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, 2,2′-azinobis (3-ethylbenzothiazoline-6-sulphonic acid) diammonium salt (ABTS), superoxide (), hydroxyl (OH•-) radicals, and Cu+2 chelating. In a wound healing assay, paravertebral excision (1 cm diameter) was made on the skin and the percentage of wound closure was measured at defined intervals (0, 3, 7, and 14 d). Results: A potent antioxidant peptide named DG-10 with the sequence DAESLSRLLG (MW: 1060.18 ± 0.5 Da) was identified from OEWPH. The peptide DG-10 showed DPPH (IC50 = 0.0085 mg/ml), ABTS•+ (IC50 = 0.56 mg/ml), superoxide (IC50 = 0.36 mg/ml), and hydroxyl (IC50 = 0.4 mg/ml) radical scavenger and copper chelating activity (IC50 = 0.28 mg/ml). In vitro cultured HFLF-pI 5, the cell model, also revealed that DG-10 could protect HFLF-pI 5 cells against H2O2-treated necrosis. Ointment composed of DG-10 peptide exhibited wound-healing properties on adult rats (Wistar strain). The percentage of wound closure in peptide-treated group was 98 by day 14. Discussion and conclusion: Our results suggested that DG-10 is a natural agent obtained from ostrich egg possessing considerable antioxidant and wound-healing properties. © 2015 Informa Healthcare USA, Inc. All rights reserved.

Item Type: Article
Additional Information: Cited By :11 Export Date: 16 February 2020 CODEN: PHBIF Correspondence Address: Asoodeh, A.; Department of Chemistry, Faculty of Sciences, Ferdowsi University of MashhadIran; email:
Uncontrolled Keywords: Bioactive peptide HFLF-PI 5 cell line HPLC DG 10 peptide egg white glutathione protein hydrolysate scavenger superoxide unclassified drug wound healing promoting agent antioxidant egg protein peptide fragment ABTS radical scavenging assay adult animal experiment animal model antioxidant activity Article chelation concentration response controlled study DPPH radical scavenging assay drug identification drug isolation drug mechanism human human cell in vitro study in vivo study male nonhuman ostrich rat reversed phase high performance liquid chromatography skin injury wound closure wound healing animal cell line cell survival dose response drug effects isolation and purification physiology pig Struthioniformes Wistar rat Rattus Struthio camelus Animals Antioxidants Dose-Response Relationship, Drug Egg Proteins Peptide Fragments Rats Rats, Wistar Swine
Subjects: QU Biochemistry
Divisions: Mashhad University of Medical Sciences
Depositing User: mr lib5 lib5
Date Deposited: 11 May 2020 07:38
Last Modified: 11 May 2020 07:38

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