Relaxant effects of different fractions from Tymus vulgaris on guinea-pig tracheal chains

Keyhanmanesh, R. and Boskabady, M. H. (2012) Relaxant effects of different fractions from Tymus vulgaris on guinea-pig tracheal chains. Biological Research, 45 (1). pp. 67-73.

[img] Text
Relaxant effects of different fractions.pdf

Download (643kB)

Abstract

In previous studies, the relaxant effect of Tymus vulgaris has been demonstrated on guinea pig tracheal chains. Therefore, in the present study, the relaxant effects of n-hexane, dichloromethane, methanol and aqueous fractions of Tymus vulgaris on tracheal chains of guinea pigs were examined. The relaxant effects of four cumulative concentrations of each fraction (0.4, 0.8, 1.2 and 1.6 g) in comparison to saline as negative control and four cumulative concentrations of theophylline (0.2, 0.4, 0.6 and 0.8 mM) were examined for their relaxant effects on precontracted tracheal chains of guinea pig by 60 mM KCl (group 1) and 10 μM methacholine (group 2, n = 7 for each group). In group 1, all concentrations of the n-hexane fraction and theophylline and three last concentrations (0.8, 1.2 and 1.6 g) of dichloromethane and two higher concentrations (1.2 and 1.6 g) of methanol fractions showed significant relaxant effects compared to that of saline (p<0.05 to p<0.001). In group 2, all concentrations of theophylline, n-hexane and dichloromethane fractions and three concentrations (0.8, 1.2 and 1.6 g) of methanol and two higher concentrations (1.2 and 1.6 g) of aqueous fractions showed significant relaxant effects compared to that of saline (p<0.05 to p<0.001). In addition, with group 1, the relaxant effect of all concentrations of all fractions except the n-hexane fraction, were significantly less than those of theophylline (p<0.05 to p<0.001). The n-hexane fraction showed higher relaxant effect than theophylline. The relaxant effect of all concentrations of the n-hexane fraction and the three last concentrations (0.8, 1.2 and 1.6 g) of dichloromethane and aqueous fractions were significantly greater in group 2 than in group 1 (p<0.05 to p<0.001). There were significant positive correlations between the relaxant effects and concentrations for theophylline and all fractions (except aqueous fraction in group 1) in both groups, but a negative correlation for the aqueous fraction in group 1 (p<0.05 to p<0.001). These results showed a potent relaxant effect for n-hexane and weaker relaxant effect for other fractions from Tymus vulgaris on tracheal chains of guinea pigs.

Item Type: Article
Additional Information: Cited By :7 Export Date: 16 February 2020 CODEN: BESEE Correspondence Address: Boskabady, M. H.; Dept. of Physiology, Medical School, Post Code 9177948564, Mashhad, Iran; email: boskabadymh@mums.ac.ir
Uncontrolled Keywords: Fractions Guinea pig Relaxant effects Trachea Tymus vulgaris bronchodilating agent dichloromethane hexane methanol n-hexane plant extract theophylline vegetable oil water animal article chemistry drug effect female male muscle relaxation phytotherapy smooth muscle solution and solubility Thymus (plant) Animals Bronchodilator Agents Guinea Pigs Hexanes Methylene Chloride Muscle, Smooth Plant Extracts Plant Oils Solutions Thymus Plant Cavia
Subjects: QV pharmacology
Divisions: Mashhad University of Medical Sciences
Depositing User: mr lib5 lib5
Date Deposited: 13 May 2020 07:20
Last Modified: 13 May 2020 07:20
URI: http://eprints.mums.ac.ir/id/eprint/18915

Actions (login required)

View Item View Item