Umbelliprenin is cytotoxic against QU-DB large cell lung cancer cell line but anti-proliferative against A549 adenocarcinoma cells

Khaghanzadeh, N. and Mojtahedi, Z. and Ramezani, M. and Erfani, N. and Ghaderi, A. (2012) Umbelliprenin is cytotoxic against QU-DB large cell lung cancer cell line but anti-proliferative against A549 adenocarcinoma cells. DARU, Journal of Pharmaceutical Sciences, 20 (1).

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Abstract

Background: Umbelliprenin is a natural compound, belonging to the class of sesquiterpene coumarins. Recently, umbelliprenin has attracted the researchers' attention for its antitumor activities against skin tumors. Its effect on lung cancer is largely unknown. The aim of our study was to investigate the effects of this natural compound, which is expected to have low adverse effects, on lung cancer. Methods. The QU-DB large cell and A549 adenocarcinoma lung cancer cell lines were treated with umbelliprenin. IC50 values were estimated using methyl thiazolely diphenyl-tetrazolium bromide (MTT) assay, in which a decrease in MTT reduction can occur as a result of cell death or cell proliferation inhibition. To quantify the rate of cell death at IC50 values, flow cytometry using Annexin V-FITC (for apoptotic cells), and propidium iodide (for necrotic cells) dyes were employed. Results: Data from three independent MTT experiments in triplicate revealed that IC50 values for QU-DB and A549 were 47 5.3 M and 52 1.97 M, respectively. Annexin V/PI staining demonstrated that umbelliprenin treatment at IC50 induced 50 cell death in QU-DB cells, but produced no significant death in A549 cells until increasing the umbelliprenin concentration to IC80. The pattern of cell death was predominantly apoptosis in both cell lines. When peripheral blood mononuclear cells were treated with 50 M and less concentrations of umbelliprenin, no suppressive effect was observed. Conclusions: We found cytotoxic/anti-proliferative effects of umbelliprenin against two different types of lung cancer cell lines. © 2012 Khaghanzadeh et al.; licensee BioMed Central Ltd.

Item Type: Article
Additional Information: Cited By :18 Export Date: 16 February 2020 CODEN: DJTSF Correspondence Address: Mojtahedi, Z.; Shiraz Institute for Cancer Research, Shiraz University of Medical Sciences, School of Medicine, Shiraz, Iran; email: mojtahediz@sums.ac.ir
Uncontrolled Keywords: Annexin IC50 Lung cancer MTT assay Propidium Iodide Umbelliprenin 3 (4,5 dimethyl 2 thiazolyl) 2,5 diphenyltetrazolium bromide coumarin derivative fluorescein isothiocyanate lipocortin 5 unclassified drug antiproliferative activity apoptosis article cancer cell culture carcinoma cell cell death cell proliferation controlled study drug cytotoxicity drug effect flow cytometry human human cell IC 50 lung adenocarcinoma
Subjects: WF Respiratory System
QZ pathology-neoplasms-Genetics
Divisions: Mashhad University of Medical Sciences
Depositing User: mr lib5 lib5
Date Deposited: 13 May 2020 07:51
Last Modified: 13 May 2020 07:51
URI: http://eprints.mums.ac.ir/id/eprint/18917

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