Effects of maternal nicotine exposure on expression of laminin alpha 5 in lung tissue of newborn

Kohbanani, M. S. and Nikravesh, M. R. and Jalali, M. and Fazel, A. and Sankian, M. and Bideskan, A. E. (2012) Effects of maternal nicotine exposure on expression of laminin alpha 5 in lung tissue of newborn. Pakistan Journal of Biological Sciences, 15 (24). pp. 1168-1175.

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Abstract

Maternal smoking has been clearly demonstrated to be associated with increased health problems in infants and children. Nicotine is the chemical substance with high level of toxicity. It crosses through the placenta and accumulates in the developing organs of fetus. Previous investigation indicated that maternal nicotine exposures induce decreased fibronectin expression in lung parenchyma. In this study, the effect of maternal nicotine exposure on laminin expression of the newborn mice lungs has been evaluated. Female pregnant Balb/C mice were divided randomly in to four groups as fallow: Experimental groupl (Exp D1); was received 3 mg kg-1 nicotine intra peritoneal injection (IP) from gestational day 7 (GD7) to the last day of pregnancy, Experimental group 2 (Exp D14); was received 3 mg kg-1 nicotine from GD7 to postnatal day 14, Groups 3 and 4; as sham control groups (Sha-Con) were received the same volume (3 mg kg-1) of normal saline parallel to experimental groups. At the end of exposure times, all of newborns were anesthetized; their lungs were removed and prepared for immunohistochemical method and real-time polymerase chain reaction. The finding indicated that laminin alpha 5 (Lama5) mRNA expressions in the lung of newborn in the nicotine treated Exp Dl decreased by 0.63 fold but increased in Exp D14 by 1.57 fold comparing to Sh-Con groups. Lama5 immunoreactivity was not similar in different parts of the lungs including alveoli and bronchiole, having a significant increase in the experimental groups in contrast to the Sh-Con groups. However, increase in immunoreactivity observed more in Exp Dl 4. Immunoreactivity intensity in small vessels of all experimental groups was not significantly different. These data also indicate that maternal nicotine exposure may induce abnormal laminin expression which may cause defects in lung function during life time. © 2012 Asian Network for Scientific Information.

Item Type: Article
Additional Information: Cited By :7 Export Date: 16 February 2020 Correspondence Address: Nikravesh, M. R.; Department of Anatomy and cell biology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
Uncontrolled Keywords: Immunohistochemistry Laminin Lung Newborn Nicotine laminin alpha5 messenger RNA animal article Bagg albino mouse biosynthesis drug effect environmental exposure female fetomaternal transfusion genetics male metabolism mouse pregnancy prenatal exposure randomization smoking Animals Animals, Newborn Maternal Exposure Maternal-Fetal Exchange Mice Mice, Inbred BALB C Prenatal Exposure Delayed Effects Random Allocation RNA, Messenger
Subjects: WP Gynecology
Divisions: Mashhad University of Medical Sciences
Depositing User: mr lib5 lib5
Date Deposited: 14 May 2020 17:37
Last Modified: 14 May 2020 17:37
URI: http://eprints.mums.ac.ir/id/eprint/18936

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