Lethal effect of blue light-activated hydrogen peroxide, curcumin and erythrosine as potential oral photosensitizers on the viability of porphyromonas gingivalis and fusobacterium nucleatum

Mahdi, Z. and Habiboallh, G. and Mahbobeh, N. N. and Mina, Z. J. and Majid, Z. and Nooshin, A. (2015) Lethal effect of blue light-activated hydrogen peroxide, curcumin and erythrosine as potential oral photosensitizers on the viability of porphyromonas gingivalis and fusobacterium nucleatum. Laser Therapy, 24 (2). pp. 103-111.

[img] Text
24_15-OR-09.pdf

Download (401kB)

Abstract

Objectives: Recently, photodynamic therapy (PDT) has been introduced as a new modality in oral bacterial decontamination. Current research aims to evaluate the effect of photodynamic killing of visible blue light in the presence of hydrogen peroxide, curcumin and erythrosine as potential oral photosensitizers on Porphyromonas gingivalis associated with periodontal bone loss and Fusobacterium nucleatum associated with soft tissue inflammation. Materials and methods: Standard suspension of P. gingivalis and F. nucleatum were exposed to Light Emitting Diode (LED) (440-480 nm) in combination with erythrosine (22 μm), curcumin (60 μM) and hydrogen peroxide (0.3 mM) for 5 min. Bacterial samples from each treatment groups (radiation-only group, photosensitizer-only group and blue light-activated photosensitizer group) were subcultured onto the surface of agar plates. Survival of these bacteria was determined by counting the number of colony forming units (CFU) after incubation. Results: Results for antibacterial assays on P. gingivalis confirmed that curcumin, Hydrogen peroxide and erythrosine alone exerted a moderate bactericidal effect which enhanced noticeably in Bconjugation with visible light. The survival rate of P. gingivalis reached zero present when the suspension exposed to blue light-activated curcumin and hydrogen peroxide for 2 min. Besides, curcumin exerted a remarkable antibacterial activity against F. nucleatum in comparison with erythrosine and hydrogen peroxide (P=0.00). Furthermore, the bactericidal effect of visible light alone on P. gingivalis as black-pigmented bacteria was significant. Conclusion: Our result suggested that visible blue light in the presence of erythrosine, curcumin and hydrogen peroxide would be consider as a potential approach of PDT to kill the main gramnegative periodontal pathogens. From a clinical standpoint, this regimen could be established as an additional minimally invasive antibacterial treatment of plaque induced periodontal pathologies. © 2015 JMLL, Tokyo, Japan

Item Type: Article
Additional Information: Cited By :16 Export Date: 16 February 2020 Correspondence Address: Mahdi, Z.2606, 35 High Park Ave, Canada; email: mahdizakery@yahoo.com
Uncontrolled Keywords: Erythrosine • Periopathogenic bacteria • Visible blue light • Curcumin • Hydrogen peroxide • Oral antibacterial activity curcumin erythrosine hydrogen peroxide alveolar bone loss antibacterial activity Article bacterial growth bacterial survival bacterial viability bactericidal activity blue light colony forming unit controlled study drug effect Fusobacterium nucleatum lethality light emitting diode nonhuman photodynamic therapy photosensitization Porphyromonas gingivalis
Subjects: WU Dentistry. Oral surgery
Divisions: Mashhad University of Medical Sciences
Depositing User: mr lib5 lib5
Date Deposited: 09 May 2020 09:19
Last Modified: 09 May 2020 09:19
URI: http://eprints.mums.ac.ir/id/eprint/18948

Actions (login required)

View Item View Item