Translation elongation factor 1-α gene as a potential taxonomic and identification marker in dermatophytes

Mirhendi, H. and Makimura, K. and De Hoog, G. S. and Rezaei-Matehkolaei, A. and Najafzadeh, M. J. and Umeda, Y. and Ahmadi, B. (2015) Translation elongation factor 1-α gene as a potential taxonomic and identification marker in dermatophytes. Medical Mycology, 53 (3). pp. 215-224.

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Abstract

Intra- and interspecies variations of the translation elongation factor 1-α (Tef - 1 α) gene were evaluated as a new identification marker in a wide range of dermatophytes, which included 167 strains of 30 species. An optimized pan-dermatophyte primer pair was designed, and the target was sequenced. Consensus sequences were used for multiple alignment and phylogenetic tree analysis and the levels of intra- and interspecific nucleotide polymorphism were assessed. Between species, the analyzed part of the Tef - 1α gene varied in length from 709 to 769 nucleotides. Significant numbers of species including Trichophyton rubrum, T. tonsurans, T. schoenleinii, T. concentricum, T. v i o - laceum, Epidermophyton floccosum, Microsporum ferrugineum, M. canis, M. audouinii, T. equinum, T. eriotrephon, and T. erinacei were invariant in Tef-1α and had sufficient barcoding distance with neighboring species. Although overall consistency was found between ITS phylogeny as the current molecular marker of dermatophytes and Tef - 1 α, a higher discriminatory power of Tef - 1 α appeared particularly useful in some clades of closely related species such as the A. vanbreuseghemii, T. rubrum, A. benhamiae, and A. otae complexes. Nevertheless, we stress that a single gene can not specify species borderlines among dermatophytes and multiple lines of evidence based on a multilocus inquiry may ascertain an incontrovertible evaluation of kinship. © The Author 2014. Published by Oxford University Press on behalf of The International Society for Human and Animal Mycology.

Item Type: Article
Additional Information: Cited By :28 Export Date: 16 February 2020 CODEN: MEMYF Correspondence Address: Mirhendi, H.; Department of Medical Parasitology and Mycology, School of Public Health, National Institute of Health Research, Tehran University of Medical SciencesIran; email: mirhendi@tums.ac.ir
Uncontrolled Keywords: Dermatophytes Identification marker Translation elongation factor 1-α elongation factor 1alpha molecular marker elongation factor 1 fungal DNA fungal protein translation elongation factor 1 alpha unclassified drug Arthroderma benhamiae Arthroderma otae Article cladistics consensus sequence dermatophyte DNA sequence Epidermophyton floccosum fungal strain genetic distance genetic transcription and translation genetic variability Microsporum Microsporum audouinii Microsporum ferrugineum nonhuman phylogenetic tree sequence analysis single nucleotide polymorphism species identification Trichophyton Trichophyton concentricum Trichophyton equinum Trichophyton eriotrephon Trichophyton interdigitale Trichophyton rubrum Trichophyton schoenleinii Trichophyton tonsurans Trichophyton violaceum Arthrodermataceae chemistry classification cluster analysis genetics human molecular genetics phylogeny DNA extraction intraspecific variation mycology polymerase chain reaction protein analysis protein purification taxonomy DNA, Fungal Genetic Variation Humans Molecular Sequence Data Peptide Elongation Factor 1 Sequence Analysis, DNA
Subjects: WC Communicable Diseases
Divisions: Mashhad University of Medical Sciences
Depositing User: mr lib5 lib5
Date Deposited: 15 May 2020 12:47
Last Modified: 15 May 2020 12:47
URI: http://eprints.mums.ac.ir/id/eprint/18971

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