In vitro antimicrobial comparison of chlorhexidine, persica mouthwash and miswak extract

Moeintaghavi, A. and Arab, H. and Khajekaramodini, M. and Hosseini, R. and Danesteh, H. and Niknami, H. (2012) In vitro antimicrobial comparison of chlorhexidine, persica mouthwash and miswak extract. Journal of Contemporary Dental Practice, 13 (2). pp. 147-152.

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Aim: Herbal mouthwashes, such as persica (Salvadora persica, mint and yarrow extracts) and miswak extract have been shown to decrease gingival inflammation and plaque accumulation. The aim of this study was to compare the antimicrobial activities of persica and miswak extract with the conventional mouthwash chlorhexidine against Streptococcus salivarius, Streptococcus sanguis, Lactobacillus vulgaris and Candida albicans. Materials and methods: In this in vitro study, blood-agar culture (Merk, Germany) was used to grow the streptococcus strains, saburd-dextrose culture (Merk, Germany) was used to grow C. albicans and MRS-agar was used to grow L. vulgaris. Various concentrations of these substances (0.1, 0.05 and 0.025 of miswak extract, 0.1, 0.05, 0.025 and 0.0125 of persica, 0.2, 0.1, 0.05 and 0.025 of chlorhexidine) were added to paper disks, separately, inserted into culture plates and transferred into the incubator. The inhibition zone around each disk was measured after 24 hours and the data was analyzed by the Kruskal-Wallis test. Results: Chlorhexidine possessed antibacterial activity at all concentrations tested. It was more effective than persica and miswak at all concentrations on S. salivarius (p = 0.022 for 0.1, 0.009 for 0.05 and 0.025). It had greater effect than the other two tested material on S. sanguis only at concentration 0.01. Chlorhexidine was the most effective against S.salivarius; persica was the most effective against Lactobacillus (p = 0.005) and the least effective against S. salivarius; and miswak extract was the most effective against S. salivarius and S. sanguis at concentrations 0.1 and 0.05 (p = 0.005) and ineffective against L. vulgaris. None of these mouthwashes were effective against C. albicans. Conclusion: This study revealed that chlorhexidine remains the gold standard as an antimicrobial agent, although herbalbased mouthwashes do have marginal antimicrobial activities. It is necessary to conduct more clinical and microbiological studies focusing on periodontal pathogens and anaerobic microorganisms. Clinical significance: Mechanical plaque control is the main way for periodontal disease prevention and mouthrinses are used to improve its efficacy. Based on the results of this study, chlorhexidine has the most antibacterial effect and although persica mouthwash and miswak are routinely used in some Asian countries their antibacterial efficacies are suspected.

Item Type: Article
Additional Information: Cited By :11 Export Date: 16 February 2020 Correspondence Address: Moeintaghavi, A.; Department of Periodontics, Dental Material Reseach Center, Faculty of Dentistry, Mashhad University of Medical SciencesIran; email:
Uncontrolled Keywords: Antimicrobial activity Chlorhexidine Miswak extract Persica mouthwash
Subjects: QV pharmacology
Divisions: Mashhad University of Medical Sciences
Depositing User: mr lib5 lib5
Date Deposited: 09 May 2020 07:20
Last Modified: 09 May 2020 07:20

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