Mitochondrial A12308G alteration in tRNALeu(CUN) in colorectal cancer samples

Mohammed, F. M. A. and Rezaee khorasany, A. R. and Mosaieby, E. and Houshmand, M. (2015) Mitochondrial A12308G alteration in tRNALeu(CUN) in colorectal cancer samples. Diagnostic Pathology, 10 (1).

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Abstract

Background: Colorectal cancer is the third most common type of cancer in men and women and the second leading cause of cancer-related deaths in the United States and UK. Colorectal cancer is strongly related to age, with almost three-quarters of cases occurring in people aged 65 or over. Pre-symptomatic screening is one of the most powerful tools for preventing colorectal cancer. Recently, the use of mitochondrial tRNA genes mutation or polymorphism patterns as a biomarker is rapidly expanding in different cancers because tRNA genes perform several functions including processing and translation which are essential components of mitochondrial protein synthesis. The aim of the present study was to find out the association of mitochondrial A12308G alteration in tRNALeu(CUN) in colorectal cancer and its usage as a new biomarker screening test. Methods: A tumor tissues from 30 patients who had colorectal cancer were selected randomly. The A12308G alteration in tRNALeu (CUN) was screened in the 30 colorectal tumor tissues. For comparison, 100 blood samples of healthy controls using PCR-sequencing methods were selected and the following results were found. Result: The A12308G, a polymorphic mutation in V-loop tRNALeu(CUN), was found in 6 Colorectal tumor tissues and 3 healthy controls. A statistical significant difference was found between cases and control regarding the association of the A12308G mutation with the colorectal tumor (P < 0.05). Conclusions: The A12308G, a polymorphic mutation in V-loop tRNALeu(CUN), could be considered as pathogenic mutation in combination with mitochondrial external conditions and other mitochondrial genes in developing different diseases especially cancers and could be used as one of the diagnostic tool. Also it seems that maybe there is relevance between A12308G mutation and other mutations that it can cause various phenotypes. © 2015 Mohammed et al.

Item Type: Article
Additional Information: Cited By :7 Export Date: 16 February 2020 Correspondence Address: Rezaee khorasany, A.R.; Mashhad University of Medical Sciences, Department of Pharmacodynamy and Toxicology, School of PharmacyIran
Uncontrolled Keywords: A12308G mutation Colorectal cancer Mitochondrial tRNALeu(CUN) Mutation leucine transfer RNA mitochondrial DNA tumor marker case control study colorectal tumor dna mutational analysis genetic screening genetics human pathology polymerase chain reaction predictive value procedures Biomarkers, Tumor Case-Control Studies Colorectal Neoplasms DNA, Mitochondrial Genetic Testing Humans Predictive Value of Tests RNA, Transfer, Leu
Subjects: WI Digestive System
QZ pathology-neoplasms-Genetics
Divisions: Mashhad University of Medical Sciences
Depositing User: mr lib5 lib5
Date Deposited: 16 May 2020 10:34
Last Modified: 16 May 2020 10:34
URI: http://eprints.mums.ac.ir/id/eprint/18984

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