Molecular characterization of pathogenic members of the genus Fonsecaea using multilocus analysis

Sun, J. and Najafzadeh, M. J. and Gerrits van den Ende, A. H. G. and Vicente, V. A. and Feng, P. and Xi, L. and de Hoog, G. S. (2012) Molecular characterization of pathogenic members of the genus Fonsecaea using multilocus analysis. PLoS ONE, 7 (8).

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Abstract

Members of the fungal genus Fonsecaea causing human chromoblastomycosis show substantial geographic structuring. Genetic identity of clinical and environmental strains suggests transmission from plant debris, while the evolutionary processes that have led to spatially separated populations have remained unexplained. Sequences of ITS, BT2, ACT1, Cdc42, Lac and HmgA were analyzed, either by direct sequencing or by cloning. Thirty-seven clinical and environmental Fonsecaea strains from Central and South America, Asia, Africa and Europe were sequenced and possible recombination events were calculated. Phylogenetic trees of Cdc42, Lac and HmgA were statistically supported, but ITS, BT2 and ACT1 trees were not. The Standardized Index of Association (IAS) did not detect recombination (IAS = 0.4778), neither did the Phi-test for separate genes. In Fonsecaea nubica non-synonymous mutations causing functional changes were observed in Lac gene, even though no selection pressures were detected with the neutrality test (Tajima D test, p>0.05). Genetic differentiation of populations for each gene showed separation of American, African and Asian populations. Strains of clinical vs. environmental origin showed genetic distances that were comparable or lower than found in geographic differentiation. In conclusion, here we demonstrated clonality of sibling species using multilocus data, geographic structuring of populations, and a low functional and structural selective constraint during evolution of the genus Fonsecaea. © 2012 Sun et al.

Item Type: Article
Additional Information: Cited By :21 Export Date: 16 February 2020 Correspondence Address: Xi, L.; Department of Dermatology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, Guangdong, China; email: xiliyan@mail.sysu.edu.cn
Uncontrolled Keywords: laccase Africa article Asia clonal variation cloning Europe evolution female Fonsecaea Fonsecaea monophora Fonsecaea nubica Fonsecaea pedrosoi fungal gene fungal strain gene function gene mutation genetic distance genetic recombination genetic selection genetic variability human Lac gene male multilocus sequence typing nonhuman nucleotide sequence phylogenetic tree population genetics South and Central America Ascomycota Cluster Analysis Evolution, Molecular Genes, Fungal Homogentisate 1,2-Dioxygenase Humans Mycological Typing Techniques Phylogeny Polymorphism, Genetic Recombination, Genetic
Subjects: WC Communicable Diseases
WR Dermatology
Divisions: Mashhad University of Medical Sciences
Depositing User: mr lib5 lib5
Date Deposited: 16 May 2020 10:58
Last Modified: 16 May 2020 10:58
URI: http://eprints.mums.ac.ir/id/eprint/19108

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