Detection of Aspergillus flavus and A. fumigatus in bronchoalveolar lavage specimens of hematopoietic stem cell transplants and hematological malignancies patients by real-time polymerase chain reaction, nested PCR and mycological assays

Zarrinfar, H. and Mirhendi, H. and Fata, A. and Khodadadi, H. and Kordbacheh, P. (2015) Detection of Aspergillus flavus and A. fumigatus in bronchoalveolar lavage specimens of hematopoietic stem cell transplants and hematological malignancies patients by real-time polymerase chain reaction, nested PCR and mycological assays. Jundishapur Journal of Microbiology, 8 (1).

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Abstract

Background: Pulmonary aspergillosis (PA) is one of the most serious complications in immunocompromised patients, in particular among hematopoietic stem cell transplants (HSCT) and patients with hematological malignancies. Objectives: The current study aimed to evaluate the incidence of PA and utility of molecular methods in HSCT and patients with hematological malignancies, four methods including direct examination, culture, nested polymerase chain reaction (PCR) and real-time PCR were performed on bronchoalveolar lavage (BAL) specimens in Tehran, Iran. Patients and Methods: During 16 months, 46 BAL specimens were obtained from individuals with allogeneic HSCT (n = 18) and patients with hematological malignancies (n = 28). Direct wet mounts with 20 potassium hydroxide (KOH) and culture on mycological media were performed. The molecular detection of Aspergillus fumigatus and A. flavus was done by amplifying the conserved sequences of internal transcribed spacer 1 (ITS1) ribosomal DNA by nested-PCR and the β-tubulin gene by TaqMan real-time PCR. Results: Seven (15.2) out of 46 specimens were positive in direct examination and showed branched septate hyphae; 11 (23.9) had positive culture including eight (72.7) A. flavus and three (27.3) A. fumigatus; 22 (47.8) had positive nested-PCR and eight (17.4) had positive real-time PCR. The incidence of invasive pulmonary aspergillosis (IPA) in these patients included proven IPA in 1 (2.2), probable IPA in 10 (21.7), possible IPA in 19 (41.3) and not IPA in 16 cases (34.8). Conclusions: The incidence of IPA in allogeneic HSCT and patients with hematological malignancies was relatively high and A. flavus was the most common cause of PA. As molecular methods had higher sensitivity, it may be useful as screening methods in HSCT and patients with hematological malignancies, or to determine when empirical antifungal therapy can be withheld. © 2015, Ahvaz Jundishapur University of Medical Sciences.

Item Type: Article
Additional Information: Cited By :13 Export Date: 16 February 2020 Correspondence Address: Fata, A.; Research Center for Skin Diseases and Cutaneous Leishmaniasis, Department of Parasitology and Mycology, Mashhad University of Medical SciencesIran; email: fataA@mums.ac.ir
Uncontrolled Keywords: Aspergillus flavus Aspergillus fumigatus Bronchoalveolar lavage Hematological malignancies Hematopoietic stem cell transplantation Invasive pulmonary aspergillosis beta tubulin internal transcribed spacer 1 ribosome DNA adult allogeneic hematopoietic stem cell transplantation Article beta tubulin gene clinical article controlled study diagnostic test accuracy study female fungal gene fungus culture fungus identification gene amplification hematologic malignancy human incidence intermethod comparison Iran ITS1 gene lung aspergillosis lung lavage male molecular diagnosis nested polymerase chain reaction nonhuman polymerase chain reaction real time polymerase chain reaction sensitivity and specificity
Subjects: WH Hemic and Lymphatic System
QX Parasitology
Divisions: Mashhad University of Medical Sciences
Depositing User: mr lib5 lib5
Date Deposited: 04 May 2020 06:07
Last Modified: 04 May 2020 06:07
URI: http://eprints.mums.ac.ir/id/eprint/19140

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