Construction and assessment of the immunogenicity and bactericidal activity of fusion protein porin A from <i>Neisseria meningitidis</i> serogroups A and B admixed with OMV adjuvant as a novel vaccine candidate

Afrough, Parviz and Asadi Karam, Mohammad Reza and Vaziri, Farzam and Behrouzi, Ava and Siadat, Seyed Davar (2020) Construction and assessment of the immunogenicity and bactericidal activity of fusion protein porin A from <i>Neisseria meningitidis</i> serogroups A and B admixed with OMV adjuvant as a novel vaccine candidate. Iranian Journal of Basic Medical Sciences, 23 (6). pp. 737-743.

[img] Text
IJBMS_Volume 23_Issue 6_Pages 737-743.pdf

Download (762kB)
Official URL: http://ijbms.mums.ac.ir/article_15497.html

Abstract

Objective(s): The porins A and B and also outer membrane vesicles (OMVs) of Neisseria meningitidis are used for vaccine purposes. In the present study, we aimed to design a new vaccine candidate based on a fusion of PorA of serogroups A and B of N. meningitidis admixed with OMV and evaluate it in an animal model.Materials and Methods: After bioinformatic studies, a fusion protein composed of porin A from both serogroups A and B of N. meningitidis was constructed, expressed, and purified by nickel resins. Extraction of OMV of N. meningitidis was performed using a chemical method. The mice were vaccinated subcutaneously in different groups with mixtures of PorA proteins, OMV, and Freundâ��s adjuvants. Then, the immune responses were measured using the ELISA method. Finally, serum bactericidal activity (SBA) procedure was applied to assay the activity of the immune responses in mice. Results: Mice received the PorA protein plus Freundâ��s adjuvant. Mice vaccinated with PorA fusion of serogroups A plus Freundâ��s adjuvant produced more IgG, IgG1, and IgG2a than combinations admixed with OMV. Furthermore, the vaccinated mice tended to direct the IgG responses toward IgG1. Sera of the mice that received PorAᚷ꺝â��s and those that received PorA㣅 produced higher bactericidal activity than the controls. Conclusion: Fusion protein porin A could be a valuable target for developing vaccines against N. meningitidis. Although, Freundâ��s adjuvant induced the strongest IgG responses, given that Freundâ��s adjuvant has no human use, and OMV is a human adjuvant, OMV could be considered in vaccine design against N. meningitidis.

Item Type: Article
Uncontrolled Keywords: Adjuvant,Fusion protein,Neisseria meningitidis,Outer membrane vesicle,Porin A
Subjects: QV pharmacology
Divisions: Journals > Iranian J Basic Medical Sciences
Depositing User: ijbms ijbms
Date Deposited: 23 Jun 2020 09:47
Last Modified: 23 Jun 2020 09:47
URI: http://eprints.mums.ac.ir/id/eprint/19283

Actions (login required)

View Item View Item