<i>Mesobuthus eupeus</i> venom induced injury in the colorectal carcinoma cell line (HT29) through altering the mitochondria membrane stability

Valizade, Massood and Raesi Vanani, Atefeh and Rezaei, Mohsen and Khorsandi, Layasadat and Zeidooni, Leila and Mahdavinia, Masoud (2020) <i>Mesobuthus eupeus</i> venom induced injury in the colorectal carcinoma cell line (HT29) through altering the mitochondria membrane stability. Iranian Journal of Basic Medical Sciences, 23 (6). pp. 760-767.

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Abstract

Objective(s): The purpose of this study was to investigate cytotoxicity and membrane toxicity effects induced by Mesobuthus eupeus venom (MEV) on the HT-29 cell line. Materials and Methods: To determine the in vitro cytotoxicity via MTT assays, HT-29 (as cancer cell line) and Hek-293T (as normal cell) were treated through different concentrations of MEV, and cytotoxicity effects were then measured through assessment of mitochondrial membrane potential (�Ψm), reactive oxygen species (ROS) generation, and apoptosis induction. The colony formation assay was performed to measure the antiproliferative effect of MEV on HT-29 cells. Nuclei alterations were also observed during apoptosis following DAPI staining. Besides, atomic force microscopy (AFM) was used to detect alterations in morphology and ultrastructure of the cells at a nanoscale level.Results: According to MTT and clonogenic assays, MEV caused a significant decrease in cell viability and proliferation of HT-29 cells while it did not have any impact on normal cells and the IC50 value was found to be 10 µg/ml. Induction of apoptosis was also confirmed by flowcytometric analysis in HT-29 cells. Moreover, the results indicated that MEV had led to a suppression of proliferation and induction of apoptosis through increased ROS and depolarization of mitochondria. Furthermore, AFM imaging demonstrated apoptosis cell death after being treated with MEV in HT-29 cells. Conclusion: This study showed that MEV had an antiproliferative effect on HT-29 cells by inducing apoptosis through the mitochondria signaling pathway. These findings suggested that MEV could be used as a promising natural remedy for cancer treatment.

Item Type: Article
Uncontrolled Keywords: AFM,Apoptosis,HT-29 cancer cell,Mesobuthus eupeus venom,Mitochondrial membrane potential,ROS
Subjects: QV pharmacology
Divisions: Journals > Iranian J Basic Medical Sciences
Depositing User: ijbms ijbms
Date Deposited: 23 Jun 2020 05:45
Last Modified: 23 Jun 2020 05:45
URI: http://eprints.mums.ac.ir/id/eprint/19286

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