Point-of-care detection of <i>Escherichia coli</i> O157:H7 in water using AuNPs-based aptasensor

Soheili, Vahid and Taghdisi, Seyed Mohammad and Abnous, Khalil and Ebrahimi, Mohsen (2020) Point-of-care detection of <i>Escherichia coli</i> O157:H7 in water using AuNPs-based aptasensor. Iranian Journal of Basic Medical Sciences, 23 (7). pp. 901-908.

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Objective(s): Access to safe drinking and irrigation water has always been one of the major human concerns worldwide. Thus, rapid, sensitive, and inexpensive approaches for pathogenic bacteria detection, such as Escherichia coli O157:H7 (EHEC) that can induce important infectious diseases, are highly on demand. Materials and Methods: In this study, a sensitive aptamer-based AuNPs bioassay was developed that demonstrated its potential to detect EHEC. In the presence of the target bacterium, the specific adsorbed aptamer, leaves AuNPs surface and interacts with EHEC. The bare nanoparticles aggregate in the presence of NaCl and the color shifts from red to purple and blue depending on the bacterial concentration. Results: The proposed aptasensor exhibited a good linear response over a wide concentration range of 876 to 107 CFU/ml and was closely correlated with the line equation of “y=0.0094x+0.0006” (R2= 0.9861). It also showed a low detection limit (LOD) of 263 CFU/ml (Signal/Noise=3). No response was recorded in the presence of other tested bacterial strains including Listeria monocytogenes and Salmonella typhi, indicating the high selectivity of the aptasensor. Conclusion: This biosensor may be used as a smart device to screen water reservoirs and prevents the outbreak of EHEC-related life-threatening contagious diseases.

Item Type: Article
Uncontrolled Keywords: Aptamer,Aptasensor,AuNPs,Escherichia coli O157:H7,water
Subjects: QU Biochemistry
Divisions: Journals > Iranian J Basic Medical Sciences
Depositing User: ijbms ijbms
Date Deposited: 16 Jun 2020 07:51
Last Modified: 16 Jun 2020 07:51
URI: http://eprints.mums.ac.ir/id/eprint/19305

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