Interaction of perforin and granzyme B and HTLV-1 viral factors is associated with ATL development

Akbarin, Mohammad Mehdi and Farhadi, Sadegh and Allahyari, Abolghasem and Koshayar, Mohammad Mehdi and Shirdel, Abbas and Rahimi, Hossain and Rezaee, Abdolrahim and Mahdifar, Maryam and Mozaheb, Zahra and Mohamadi, Asadollah and Bari, Alireza and Mohaddes, Seyedeh Tahereh and Rafatpanah, Houshang (2020) Interaction of perforin and granzyme B and HTLV-1 viral factors is associated with ATL development. Iranian Journal of Basic Medical Sciences, 23 (8). pp. 1007-1011.

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Abstract

Objective(s): Human T cell leukaemia virus type 1 (HTLV-1) is associated with adult T cell leukaemia (ATL), a malignant lymphoproliferative disease that infects CD4 T cells. It is not clear why the majority of HTLV-1-infected individuals remain asymptomatic carries (ACs) and a minority develop ATL. Cellular immune response has a critical role in ATL and destroys malignant and HTLV-1-infected cells. Perforin and granzyme have important functional roles in apoptosis and destruction of infected cells. In the present study we examined the role of perforin and granzyme in ATL patients and ACs.Materials and Methods: Peripheral blood mononuclear cells (PBMCs) were isolated from ATL patients and ACs by using Ficoll-hypaque density centrifugation. RNA was extracted and cDNA was synthesized. A real-time PCR TaqMan method was designed and optimized for evaluation of perforin, granzyme, tax, and HBZ gene expression. HTLV-1 proviral load (PVL) was quantified in patients with ATL and ACs.Results: The mRNA expression of tax and HBZ was significantly higher in ATL patients than ACs (P=0.011 and P=0.0001,respectively). The HTLV-1 PVL was higher in ATL patients compared to with AC group (P=0.015). There was a significant increase in perforin gene expression in ACs compared with ATL patients (P=0.002). Furthermore, the expression of granzyme was also higher in ACs compared with ATL patients, and significant differences were observed between the two groups (P=0.036).Conclusion: Low expression of perforin and granzyme in ATL patients seems to influence the efficiency of CTL function and destruction of HTLV-1-infected cells, which might contribute to the disease pathogenesis.

Item Type: Article
Uncontrolled Keywords: ATL Granzyme HTLV,1 Perforin Proviral load
Subjects: QV pharmacology
Divisions: Journals > Iranian J Basic Medical Sciences
Depositing User: ijbms ijbms
Date Deposited: 28 Jun 2020 09:46
Last Modified: 28 Jun 2020 09:46
URI: http://eprints.mums.ac.ir/id/eprint/19388

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