Bioinformatics prediction and experimental validation of VH antibody fragment interacting with <i>Neisseria meningitidis</i> factor H binding protein

Rafighdoust, Hediyeh and Ahangarzadeh, Shahrzad and Yarian, Fatemeh and Taheri, Ramezan Ali and Lari, Arezou and Bandehpour, Mojgan and Salahshoor Dahr, Mona (2020) Bioinformatics prediction and experimental validation of VH antibody fragment interacting with <i>Neisseria meningitidis</i> factor H binding protein. Iranian Journal of Basic Medical Sciences, 23 (8). pp. 1053-1058.

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Abstract

Objective(s): We previously conducted an in silico research on the interactions between the ribosome display-selected single chain variable fragment (scFv) and factor H binding protein (fHbp) of Neisseria meningitidis. We found that heavy chain variable (VH) fragment of this scFv had considerable affinity to fHbp. These results led us to evaluate the ability of this small antibody fragment in binding and detection of fHbp antigen.Materials and Methods: In this study, at first, the three-dimensional structure of VH fragment was simulated by Kotai Antibody Builder web server. By using ClusPro 2.0 web server, the 3D structure of the soluble form of fHbp (PDB: 2KC0) was docked to the modeled VH fragment to extract the structure of the complex�s binding. Molecular dynamics (MD) simulation was carried out using GROMACS 4.5.3 package for 65 ns. Secondly, coding sequence of VH fragment was cloned separately and expressed in Escherichia coli. After purification of the VH fragment, its binding activity to fHbp protein was analyzed by enzyme-linked immunosorbent assay (ELISA) and surface plasmon resonance (SPR) method.Results: Important amino acids involved in antigen- antibody interaction were identified by analyzing the fHbp-VH complex. The ability of the VH antibody fragment to bind and detect fHbp antigen has been confirmed by the results of in silico analysis, ELISA and SPR methods.Conclusion: These results showed that this small fragment of antibody could be used for designing diagnostic kits.

Item Type: Article
Uncontrolled Keywords: Cloning,Heavy chain variable,Modeling,Molecular docking,Surface Plasmon Resonance
Subjects: QV pharmacology
Divisions: Journals > Iranian J Basic Medical Sciences
Depositing User: ijbms ijbms
Date Deposited: 28 Jun 2020 09:50
Last Modified: 28 Jun 2020 09:50
URI: http://eprints.mums.ac.ir/id/eprint/19394

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