Evaluation of D-isomer of 18F-FBPA for oncology PET focusing on the differentiation of glioma and inflammation

Hirai, Nobuto and Watabe, Tadashi and Nagamori, Shushi and Wiriyasermkul, Pattama and Tanaka, Yoko and Romanov, Victor and Naka, Sadahiro and Kanai, Yasukazu and Liu, Yuwei and Tani, Naoki and Sakai, Tatsuya and Tatsumi, Mitsuaki and Shimosegawa, Eku and Kanai, Yoshikatsu and Hatazawa, Jun (2020) Evaluation of D-isomer of 18F-FBPA for oncology PET focusing on the differentiation of glioma and inflammation. Asia Oceania Journal of Nuclear Medicine and Biology, 8 (2). pp. 102-108.

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Objective(s): L-4-borono-2-18F-fluoro-phenylalanine (L-18FFBPA), a substrate of L-type amino acid transporter 1 (LAT1), is a tumor-specific probe used in positron emission tomography (PET). On the other hand, it has not been examined whether another isomer D-18FFBPA accumulates specifically in the tumor. Here, we compared the accumulation of D-18FFBPA in C6 glioma and inflammation to evaluate the performance of D-18FFBPA as a tumor-specific probe. Methods: HEK293-LAT1 and HEK293-LAT2 cells were tested for 14C-leucine or 14C-alanine transport, and IC50 values of L- and D-FBPA were evaluated in both cell types. PET was conducted in rat xenograft model of C6 glioma with LAT1 expression and model of turpentine oil-induced subcutaneous inflammation (n=10 for both models). The concentrations of D-18FFBPA were compared between glioma and inflammatory lesion using standardized uptake value (SUV). Results: In contrast to L-FBPA, which inhibited substrate uptake in both HEK293-LAT1 and -LAT2 cells, D-FBPA showed no inhibitory effect on both cells, suggesting low transporter selectivity of D-18FFBPA against LAT1 and LAT2. Static PET analysis showed low accumulation of D-18FFBPA in C6 glioma and inflammatory lesion (SUVmax=0.80±0.16, 0.56±0.09, respectively). Although there was a statistical difference in SUVmax between these tissues, it was difficult to distinguish glioma from inflammation on the PET image due to its low uptake level. Therefore, it was suggested that D-18FFBPA is not a suitable tumor-specific probe for oncology PET in contrast to L-18FFBPA. Conclusion: This study demonstrated that D-18FFBPA is not a LAT1-specific PET probe and shows low uptake in C6 glioma, indicating its unsuitability as a tumor diagnosis PET probe.

Item Type: Article
Uncontrolled Keywords: FBPA,small animal PET,LAT1,C6 glioma,Inflammation
Subjects: WN Radiology . Diagnostic Imaging
Divisions: Journals > Asia Oceania J Nuclear Medicine & Biology
Depositing User: AOJNMB AOJNMB
Date Deposited: 27 Jun 2020 08:45
Last Modified: 27 Jun 2020 08:45
URI: http://eprints.mums.ac.ir/id/eprint/19466

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