Investigation of codons 164 and 27 of beta 2-adrenergic receptor gene polymorphism in women with the polycystic ovary syndrome

Zangeneh, farideh and Naghizadeh, mohamad mahdi and Jafarian, tayebeh and Nejad Fateh Moghadam, saeedeh and Salmani, Arash (2016) Investigation of codons 164 and 27 of beta 2-adrenergic receptor gene polymorphism in women with the polycystic ovary syndrome. The Iranian Journal of Obstetrics, Gynecology and Infertility, 18 (181). pp. 1-9.

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Abstract

Introduction: Polycystic ovary syndrome (PCOS) is a common and complex disease in women. Although the role of genetic factors in PCOS is strongly supported, the genes that are involved in the etiology of this syndrome have not yet been fully investigated. The aim of this study was the investigation of codons 164 and 27 of beta 2-adrenergic receptor gene polymorphism in women with PCOS. Methods: This cross-sectional study was performed in 2014 on 100 women with PCOS in Imam Khomeini Hospital, Tehran, Iran. 3 ml blood samples were collected for extraction of genomic DNA by the Qiagen DNA extraction kit, then gene bank based primers were prepared for PCR. Approved DNA samples were sequenced by Macrogen (row or sequence); resulting sequences were evaluated by the Chromas (ver. 2.4) application. The data were analyzed using the bootstrap method and SPSS software (ver.18). P < 0.05 was considered significant. Findings: The analysis of polymorphism in codon 164 in PCO women showed 44.4 statistically significant polymorphism (P<0.002). Codon 27 did not show any significant polymorphism differences between PCO and non-PCO groups (P=0.606). Homozygous and heterozygous in both codons were significantly different (P=0.004 and P=0.0450, respectively). Conclusion: Inspection of beta2 adrenoceptor gene polymorphism in codons 164 and 27 showed that codon 164 is associated with PCO.

Item Type: Article
Subjects: WP Gynecology
WQ Obstetrics
Divisions: Journals > Iranian J Obstetrics, Gynecology and Infertility
Depositing User: ijogi ijogi
Date Deposited: 21 Sep 2017 14:29
Last Modified: 21 Sep 2017 14:29
URI: http://eprints.mums.ac.ir/id/eprint/2211

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