CFP10: mFcγ2 as a novel tuberculosis vaccine candidate increases immune response in mouse

Baghani, Ali Asghar and Soleimanpour, Saman and Farsiani, Hadi and Mosavat, Arman and Yousefi, Masoud and Meshkat, Zahra and Rezaee, Seyed Abdolrahim and Amel Jamehdar, Saeid and Akbari Eydgahi, Mohammad Reza and Sadeghian, Hamid and Ghazvini, Kiarash (2017) CFP10: mFcγ2 as a novel tuberculosis vaccine candidate increases immune response in mouse. Iranian Journal of Basic Medical Sciences, 20 (2). pp. 122-130.

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Official URL: http://ijbms.mums.ac.ir/article_8231.html

Abstract

Objective(s): Despite treatment with antibiotics and vaccination with BCG, tuberculosis (TB) is still considered as one of the most important public health problems in the world. Therefore, designing and producing a more effective vaccine against TB seems urgently. In this study, immunogenicity of a fusion protein which consisting or comprising CFP-10 from Mycobacterium tuberculosis and the Fc-domain of mouse IgG2a was evaluated as a novel subunit vaccine candidate against TB. Materials and Methods: The genetic constructs were cloned in pPICZαA expression vector and recombinant vectors (pPICZαA-CFP-10: Fcγ2a and pPICZαA-CFP-10:His) were transformed into Pichia pastoris. To evaluate the expression of recombinant proteins, SDS-PAGE and immunoblotting were used. The immunogenicity of recombinant proteins, with and without BCG were assessed in BALB/c mice and specific cytokines against recombinant proteins (IFN-γ, IL-12, IL-4, IL-17 and TGF-β) were evaluated. Results: The levels of IFN-γ and IL-12 in mice that received recombinant proteins was higher than the control groups (BCG and PBS). Thus, both recombinant proteins (CFP-10:Fcγ2a and CFP-10:His) could excite good response in Th1-cells. The Fc-tagged protein had a stronger Th1 response with low levels of IL-4, as compared to CFP-10:His. However, the highest level of Th1 response was observed in groups that were vaccinated with BCG (prime) and then received recombinant protein CFP-10: Fcγ2a (booster). Conclusion: The results demonstrated that binding mice Fc-domain to CFP-10 protein can increase the immunogenicity of the subunit vaccine. Further studies, might be able to design and produce a new generation of subunit vaccines based on the Fc-fused immunogen.

Item Type: Article
Subjects: QW Microbiology and Immunology
Divisions: Journals > Iranian J Basic Medical Sciences
Depositing User: ijbms ijbms
Date Deposited: 24 Sep 2017 14:19
Last Modified: 24 Sep 2017 14:19
URI: http://eprints.mums.ac.ir/id/eprint/2552

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