Effect of Ganoderma lucidum hydroalcoholic extract on insulin release in rat-isolated pancreatic islets

Shafiee-Nick, Reza and Parizadeh, Seyyed Mohammad Reza and Zokaei, Nona and Ghorbani, Ahmad (2012) Effect of Ganoderma lucidum hydroalcoholic extract on insulin release in rat-isolated pancreatic islets. Avicenna Journal of Phytomedicine, 2 (4). pp. 206-211.

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Abstract

Objective: Ganoderma Lucidum (G. Lucidum) has been suggested to increase serum insulin level.This study was undertaken to investigateits direct effect on the islets of Langerhans. Material and Methods: Male albino Wistar rats were anesthetized and the islets were isolated after digestion of the pancreas with collagenase. The islets were incubated for 60 min in Krebs bicarbonate buffer containing 3 or 10 mM glucose in the presence of hydroalcoholic extract of G. Lucidum (1 mg/ml), 3-isobutyl-1-methylxanthine (IBMX, 100 µM) or vehicle. Results: Exposure of islets to the extract increased insulin secretion at basal (3 mM) glucose concentration. Increase of glucose concentration to 10 mM resulted in a significant increase in the rate of insulin secretion. While the IBMX could augment insulin release evoked by 10 mM glucose, the extract failed to modify it. Conclusion: Our results demonstrate that G. lucidum acts directly on the Langerhans islets to increase basal insulin release.

Item Type: Article
Subjects: WI Digestive System
QU Biochemistry
Divisions: Journals > Avicenna J Phytomedicine
Depositing User: ajp ajp
Date Deposited: 23 Sep 2017 17:18
Last Modified: 23 Sep 2017 17:18
URI: http://eprints.mums.ac.ir/id/eprint/2953

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