Azimi, Leila and Fallah, Fatemeh and Karimi, Abdollah and Shirdoust, Mehdi and Azimi, Taher and Sedighi, Iraj Sedighi and Rahbar, Mohammad and Armin, Shahnaz (2020) Survey of various carbapenem-resistant mechanisms of <i>Acinetobacter baumannii</i> and <i>Pseudomonas aeruginosa</i> isolated from clinical samples in Iran. Iranian Journal of Basic Medical Sciences, 23 (11). pp. 1396-1400.
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Abstract
Objective(s): Pseudomonas aeruginosa and Acinetobacter baumannii resist antibiotics by different intrinsic and acquired mechanisms. This study aims to define various carbapenem-resistant mechanisms of isolated P. aeruginosa and A. baumannii from nine different provinces of Iran.Materials and Methods: In this cross-sectional study, all carbapenem-resistant P. aeruginosa and A. baumannii samples from nine provinces of Iran on a one-year time horizon were gathered. Modified Hedge Test (MHT) and Carba NP-Test were applied to the identification of producing-carbapenemase strains. The most important carbapenemase genes recognized by PCR and gene overexpression of the efflux pump were surveyed by efflux pump inhibitors (EPIs) and confirmed by Real-Time PCR. Results: Twenty-one percent and 43.5 of P. aeruginosa and A. baumannii isolates were resistant to carbapenem, respectively. MHT and Carba-NP tests identified 21 and 11 carbapenemase-producing strains in these Gram-negative bacteria, respectively. NDM-1 was the most prevalently detected carbapenemase in P. aeruginosa; OXA-51 and OXA-23 were the most significant genes in A. baumannii. EPIs identified active efflux pumps in 20 and 28 of P. aeruginosa and A. baumannii, respectively. Real-time PCR confirmed gene overexpression of efflux pumps in 54 and 30 of positive EPIs in P. aeruginosa and A. baumannii, respectively. Conclusion: P. aeruginosa and A. baumannii may become multi-drug-resistant (MDR) and Extensively Drug-Resistant (XDR) strains and cause a high rate of mortality and morbidity. Thus, it is of necessity to prohibit the spread of antibiotic-resistant strains in hospitals.
Item Type: | Article |
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Uncontrolled Keywords: | Acinetobacter baumannii,Carbapenems,Drug resistance,Iran,Pseudomonas aeruginosa |
Subjects: | QV pharmacology |
Divisions: | Journals > Iranian J Basic Medical Sciences |
Depositing User: | ijbms ijbms |
Date Deposited: | 06 Sep 2021 08:52 |
Last Modified: | 06 Sep 2021 08:52 |
URI: | http://eprints.mums.ac.ir/id/eprint/37024 |
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