Cytotoxic effect of amalgam and mineral trioxide aggregate on L929 cell line culture

Tavakol Afshari, Jalil and Ghoddusi, Jamileh and Behforuzi, Elham and Khalili, Mitra (2005) Cytotoxic effect of amalgam and mineral trioxide aggregate on L929 cell line culture. Journal of Mashhad Dental School, 29 (Issue). pp. 177-186.

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Abstract

Introduction: In many cases of endodontic treatment like retrograde surgery and perforation correction, because the materials are placed adjacent to vital connective tissue, biocompatibility and non cytotoxicity are of great importance and improve healing process. Among so many introduced materials, amalgam has been used for many years as a low cytotoxic material. Mineral Trioxide Aggregate (MTA) has been introduced as a new material with high biocompatibility and cementogenesis stimulation. The purpose of this study was the comparison of the cytotoxicity of amalgam and MTA on L929 cell culture using light microscope. Materials & Methods: Different dilutions of fresh and set materials were placed adjacent to flasks of L929 in DMEM media for 24h, 48h, 72h and 4 and 6 days. Then qualitative evaluation of cell morphology by light microscope were done. Results: In qualitative evaluation on L929 cells with light microscope, fresh-neat amalgam had a moderate cytotoxic effect as proliferation inhibition, whereas fresh MTA had a low cytotoxic effect. Set materials had no cytotoxic effects. Conclusion: According to our findings, MTA revealed a lower degree of cytotoxicity than amalgam. Thus MTA could be used nearby the vital connective tissue. Key words: Amalgam, mineral trioxide aggregate, tissue cytotoxicity, L929 cell lines culture.

Item Type: Article
Subjects: WU Dentistry. Oral surgery
Divisions: Journals > Journal of Mashhad Dental School
Depositing User: jmds jmds
Date Deposited: 25 Sep 2017 13:46
Last Modified: 25 Sep 2017 13:46
URI: http://eprints.mums.ac.ir/id/eprint/3808

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