G-CSF for mobilizing transplanted bone marrow stem cells in rat model of Parkinson's disease

Safari, Manouchehr and Jafari, Behnaz and Zarbakhsh, Sam and Sameni, Hamidreza and Vafaei, Abbas Ali and Khan Mohammadi, Nasrin and Ghahari, Laya (2016) G-CSF for mobilizing transplanted bone marrow stem cells in rat model of Parkinson's disease. Iranian Journal of Basic Medical Sciences, 19 (12). pp. 1318-1324.

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Abstract

Objective(s): Granulocyte-colony stimulating factor (G-CSF) is used in clinical practice for the treatment of neutropenia and to stimulate generation of hematopoietic stem cells in bone marrow donors. In the present study, the ability of G-CSF in mobilizing exogenous bone marrow stem cells (BMSCs) from peripheral blood into the brain was tested. We for the first time injected a small amount of BMSCs through the tail vein. Materials and Methods: We choose 25 male Wistar rats (200–250 g) were lesioned by 6-OHDA injected into the left substantia nigra, pars compacta (SNpc). G-CSF (70 µg/kg/day) was given from the 7th day after lesion for five days. The BMSCs (2×105) were injected through the dorsal tail vein on the 7th day after lesion. Results:The number of rotations was significantly lower in the stem cell therapy group than in the control group. In the third test in the received G-CSF and G-CSF+stem cells groups, animals displayed significant behavioral recovery compared with the control group (P<0.05). There was a significant difference in the average of dopaminergic neurons in SNpc between the control group and G-CSF and G-CS+stem cells groups. We didn't detect any labeling stem cells in SNpc. Conclusion: G-CSF can't mobilize low amounts of exogenous BMSCs from the blood stream to injured SNpc. But G-CSF (70 µg/kg) is more neuroprotective than BMSCs (2×105 number of BMSCs). Results of our study suggest that G-CSF alone is more neuroprotective than BMSCs.

Item Type: Article
Subjects: QV pharmacology
Divisions: Journals > Iranian J Basic Medical Sciences
Depositing User: ijbms ijbms
Date Deposited: 26 Sep 2017 17:22
Last Modified: 26 Sep 2017 17:22
URI: http://eprints.mums.ac.ir/id/eprint/4813

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