Production of specific IgY antibody to the recombinant FanC protein produced in Escherichia coli

Nasiri, Khadijeh and Zibaee, Saeed and Nassiri, Mohammadreza and Tahmoorespur, Mojtaba and Haghparast, Alireza (2016) Production of specific IgY antibody to the recombinant FanC protein produced in Escherichia coli. Iranian Journal of Basic Medical Sciences, 19 (8). pp. 883-889.

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Abstract

Objective(s): Enterotoxigenic Escherichia coli (ETEC) strains are one of the primary causes of diarrhea in newborn calves and in humans, pigs, and sheep. IgY technology has been identified as a promising alternative to generating a mass amount of specific antibody for use in immunotherapy and immunodiagnostics. The purpose of this study was to produce specific antibody by egg yolk antibody (IgY) to recombinant FanC protein from ETEC. Materials and Methods: FanC (K99) gene was amplified from ETEC by specific primers and polymerase chain reaction. The gene was cloned and subcloned into pTZ57R/T and pET32a (+) vectors, respectively. Recombinant vector was transferred into E. coli BL21 CodonPlus (DE3). Protein expression was investigated by 1 mM IPTG induction. Hens were immunized by the purified recombinant FanC protein. The activity and specificity of the IgY antibody were detected by dot-blotting, Western blotting, and indirect ELISA. Results: We obtained FanC specific IgYs by immunizing the hens with the recombinant FanC protein. The anti-FanC IgY showed binding specifically to the FanC protein of ETEC. Conclusion: The results emphasize that specific IgY against the recombinant FanC protein could be recommended as a candidate for passive immunization against ETECinfection in animals and humans.

Item Type: Article
Subjects: QW Microbiology and Immunology
Divisions: Journals > Iranian J Basic Medical Sciences
Depositing User: ijbms ijbms
Date Deposited: 27 Sep 2017 17:40
Last Modified: 27 Sep 2017 17:40
URI: http://eprints.mums.ac.ir/id/eprint/5664

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