Molecular Analysis and Comparison of Y chromosome Microdeletions in Tabriz and Kashan Infertile Men with Azoospermia and Severe Oligospermia

Torfeh, Mahnaz and Sakhinia, Ebrahim and Hasani, Hasan and Ahmadi Asre Badr, Yadollah and Nourizadeh, Davoud and Heshmat, Yaghoub and Rohani, Mahdi (2012) Molecular Analysis and Comparison of Y chromosome Microdeletions in Tabriz and Kashan Infertile Men with Azoospermia and Severe Oligospermia. The Iranian Journal of Obstetrics, Gynecology and Infertility, 15 (2). pp. 22-28.

Full text not available from this repository.
Official URL: http://ijogi.mums.ac.ir/article_5740.html

Abstract

Introduction: Studies has shown that approximately 10-15 of married couples have infertility problem and a male factor can be accounted for half of these cases. Deletion in three non- overlapping regions on long arm of Y chromosome (AZFa, AZFb and AZFc) causes defective spermatogenesis and ultimately infertility in men. According to recent studies males who have Y chromosome deletions and they are by ICSI for infertility may transmit their deletions to a son offspring. So before ICSI all such men should undergo screening for the presence of these deletions in order to prevent the transmission to their male offspring. Methods: Screening of Y chromosome micro deletions was done in 100 infertile men who came to infertility center of Beheshti hospital in Kashan and infertility centers in Tabriz for ICSI. Genomic DNA was extracted from peripheral blood lymphocytes by standard ethanol chloroform techniques. By using 7 STS markers based on EAA/EMQN guideline and 11 STS markers which used in Iran and neighbor countries, PCR performed on each samples for detection of micro deletions. Results: In Kashani participants, none of the patients had these deletions, but in Tabrizi patients 4 (8) had these deletions. Conclusion: This study failed to find greatly deletion in the patients who referred for ICSI in our study, so it appears that other factors were important in the infertility in this population.

Item Type: Article
Subjects: WP Gynecology
WQ Obstetrics
Divisions: Journals > Iranian J Obstetrics, Gynecology and Infertility
Depositing User: ijogi ijogi
Date Deposited: 27 Sep 2017 18:15
Last Modified: 27 Sep 2017 18:15
URI: http://eprints.mums.ac.ir/id/eprint/5810

Actions (login required)

View Item View Item