A novel label-free cocaine assay based on aptamer-wrapped single-walled carbon nanotubes

Taghavi, Sahar and Ayatollahi, Sara and Alibolandi, Mona and Lavaee, Parirokh and Ramezani, Mohammad and Abnous, Khalil (2014) A novel label-free cocaine assay based on aptamer-wrapped single-walled carbon nanotubes. Nanomedicine Journal, 1 (2). pp. 100-106.

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Abstract

Objective(s): This paper describes a selective and sensitive biosensor based on the dissolution and aggregation of aptamer wrapped single-walled carbon nanotubes. We report on the direct detection of aptamer–cocaine interactions, namely between a DNA aptamer and cocaine molecules based on near-infrared absorption at λ807. Materials and Methods: First a DNA aptamer recognizing cocaine was non-covalently immobilized on the surface of single walled carbon nanotubes and consequently dissolution of SWNTs was occurred. Vis-NIR absorption (A807nm) of dispersed, soluble aptamer-SWNTs hybrid, before and after incubation with cocaine was measured using a CECIL9000 spectrophotometer. Results: This carbon nanotube setup enabled the reliable monitoring of the interaction of cocaine with its cognate aptamer by aggregation of SWNTs in the presence of cocaine. Disscusion: This assay system provides a mean for the label-free, concentration-dependent, and selective detection of cocaine with an observed detection limit of 49.5 nM.

Item Type: Article
Subjects: QT physiology
Divisions: Journals > Nanomedicine Journal
Depositing User: nmj nmj
Date Deposited: 28 Sep 2017 13:59
Last Modified: 28 Sep 2017 13:59
URI: http://eprints.mums.ac.ir/id/eprint/5883

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