Analysis of Sex Chromosomes Disorders in Preimplantation Genetic Diagnosis

Sahebkashaf, Hamid and Aleyasin, Ahmad and Keynejad, Paris and Sahebkashaf, Saeed and Jahanshad, Faezeh and Mohammadi Ziazi, Leila (2007) Analysis of Sex Chromosomes Disorders in Preimplantation Genetic Diagnosis. The Iranian Journal of Obstetrics, Gynecology and Infertility, 10 (2). pp. 127-132.

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Abstract

Introduction: Preimplantation genetic diagnosis (PGD) is an advanced diagnostic tool in the assisted reproductive technologies (ARTs) to avoid inheritance of genetic disease by transferring unaffected embryos. The presence of numerical sex chromosome abnormalities was studied using fluorescence in situ hybridization (FISH) with two sex chromosome specific probes for X and Y chromosomes. Material and Methods: In this experimental study, PGD has been performed for 10 couples, who were carriers of inherited X linked disorders. Conventional hormone therapy, intracytoplasmic sperm injection, blastomer, biopsy and fluorescent in situ hybridization were carried out for all candidates. Results: Data was analyzed by descriptive statistics. In FISH analysis 93 of cells provides clear signals for X and Y chromosomes from that 29(XX), 18(XY), 4(XXY), 1(XO) were diagnosed. Transferring of embryos into uterus was performed in 10 cycles resulting into two pregnancies and one born baby. The implantation rate of embryos was 20 and the take home baby rate was 10 following ten PGD experiments. Conclusion: Chromosomal disorders more frequently in embryos with dismorphology especially in the embryos from older women.

Item Type: Article
Subjects: WP Gynecology
WQ Obstetrics
Divisions: Journals > Iranian J Obstetrics, Gynecology and Infertility
Depositing User: ijogi ijogi
Date Deposited: 28 Sep 2017 15:19
Last Modified: 28 Sep 2017 15:19
URI: http://eprints.mums.ac.ir/id/eprint/6289

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