Rahmanifar, Farhad and Tamadon, Amin and Mehrabani, Davood and Zare, Shahrokh and Abasi, Sorush and Keshavarz, Saeideh and Dianatpour, Mehdi and Khodabandeh, Zahra and ghian Jahromi, Iman Raze and Koohi-Hoseinabadi, Omid (2016) Histomorphometric evaluation of treatment of rat azoosper-mic seminiferous tubules by allotransplantation of bone marrow-derived mesenchymal stem cells. Iranian Journal of Basic Medical Sciences, 19 (6). pp. 653-661.
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Abstract
Objective(s): Bone marrow-derived mesenchymal stem cells (BM-MSCs) potentials make them appropriate for cell therapy including ability of differentiation and release of anti-inflammatory cytokines and growth factors secreta. For treatment of azoospermia to induce proliferation and differentiation of germ cells, MSCs transplantation has been introduced. The aim of the present experimental case-control study was to histomorphometric evaluation of the germinal cells in seminiferous tubules of azoospermic rats before and after BM-MSCs allotransplantation. Materials and Methods: In the present study, BM-MSCs were isolated from six male rats and confirmed. Their testes also served as intact negative controls. The recipient rats (n=6) were received two doses of 10 mg/kg of busulfan with 21 days interval to induce azoospermia. After cessation of spermatogenesis, the rats were allotransplanted with the BM-MSCs into efferent duct of right testes. Thirty-five days later, the right cell-treated testes were compared to left azoospermic ones. Results: Histomorphometric analyses showed that the seminiferous tubules treated with BM-MSCs had normal morphology in comparison with azoospermic testes, which were without germinal layer. In most BM-MSCs-treated seminiferous tubules, spermatogenesis was observed. Conclusion: The allotransplanted BM-MSCs could induce spermatogenesis in seminiferous tubules of azoospermic rats.
Item Type: | Article |
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Subjects: | QV pharmacology |
Divisions: | Journals > Iranian J Basic Medical Sciences |
Depositing User: | ijbms ijbms |
Date Deposited: | 28 Sep 2017 15:20 |
Last Modified: | 28 Sep 2017 15:20 |
URI: | http://eprints.mums.ac.ir/id/eprint/6363 |
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