Umbelliprenin induced both anti-inflammatory and regulatory cytokines in C57/BL6 mice

Khaghanzadeh, Narges and Samiei, Afshin and Mojtahedi, Zahra and Ramezani, Mohammad and Hosseinzadeh, Massood and Ghaderi, Abbas (2017) Umbelliprenin induced both anti-inflammatory and regulatory cytokines in C57/BL6 mice. Iranian Journal of Basic Medical Sciences, 20 (7). pp. 829-834.

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Abstract

Objective(s): Umbelliprenin is a prenyloxy-coumarin with pharmacologically polyvalent activity. Several studies have shown its anti-inflammatory, anti-tumor, antioxidant, and antigenotoxic activity, and other functions. However, the exact mechanism of action of this compound on the immune response has not yet been shown. Here, we investigated umbelliprenin effects on the predominance of Th1 and Th2 responses in normal C57/BL6 mice. Materials and Methods: Umbelliprenin (2.5 mg/200 µl IP) were administered to six C57/BL6 mice every other day for 8 days. Paraffin and PBS-injected mice were enrolled as solvent and control groups, respectively (n=6 mice/group). IL-10, IFN-γ, and IL-4 levels were determined in sera and also in splenocytes culture supernatants in the presence of Con A (3 µg/ml) after 72 hr. H&E staining of paraffin embedded blocks was performed for lung and liver tissues of mice. Results: Umbelliprenin could significantly increase the secretion of IFN-γ and IL-4 in sera and IL-10 in splenocytes cultures. Comparison of IFN-γ /IL-4 in the sera and splenocytes culture supernatants showed lower ratios in umbelliprenin treated mice than in solvent and untreated groups. Conclusion: The in vivo study showed that umbelliprenin could induce anti-inflammatory responses via the predominance of Th2 cells and some regulatory responses in C57/BL6 mice.

Item Type: Article
Subjects: QV pharmacology
QW Microbiology and Immunology
Divisions: Journals > Iranian J Basic Medical Sciences
Depositing User: ijbms ijbms
Date Deposited: 18 Sep 2017 12:32
Last Modified: 18 Sep 2017 12:32
URI: http://eprints.mums.ac.ir/id/eprint/641

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