Berberine suppresses migration of MCF-7 breast cancer cells through down-regulation of chemokine receptors

Ahmadiankia, Naghmeh and Kalalian Moghaddam, Hamid and Mishan, Mohammad Amir and Bahrami, Ahmad Reza and Naderi-Meshkin, Hojjat and Bidkhori, Hamid Reza and Moghaddam, Maryam and Mirfeyzi, Seyed Jamal Aldin (2016) Berberine suppresses migration of MCF-7 breast cancer cells through down-regulation of chemokine receptors. Iranian Journal of Basic Medical Sciences, 19 (2). pp. 125-131.

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Abstract

Objective(s): Berberine is one of the main alkaloids and it has been proven to have different pharmacological effects including inhibition of cell cycle and progression of apoptosis in various cancerous cells; however, its effects on cancer metastasis are not well known. Cancer cells obtain the ability to change their chemokine system and convert into metastatic cells. In this study, we examined the effect of berberine on breast cancer cell migration and its probable interaction with the chemokine system in cancer cells. Materials and Methods: The MCF-7 breast cancer cell line was cultured, and then, treated with berberine (10, 20, 40 and 80 μg/ml) for 24 hr. MTT assay was used in order to determine the cytotoxic effect of berberine on MCF-7 breast cancer cells. Wound healing assay was applied to determine the inhibitory effect of berberine on cell migration. Moreover, real-time quantitative PCR analysis of selected chemokine receptors was performed to determine the probable molecular mechanism underlying the effect of berberine on breast cancer cell migration. Results: The results of wound healing assay revealed that berberine decreases cell migration. Moreover, we found that the mRNA levels of some chemokine receptors were reduced after berberine treatment, and this may be the underlying mechanism for decreased cell migration. Conclusion: Our results indicate that berberine might be a potential preventive biofactor for human breast cancer metastasis by targeting chemokine receptor genes.

Item Type: Article
Subjects: QT physiology
Divisions: Journals > Iranian J Basic Medical Sciences
Depositing User: ijbms ijbms
Date Deposited: 29 Sep 2017 02:34
Last Modified: 29 Sep 2017 02:34
URI: http://eprints.mums.ac.ir/id/eprint/6642

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