Antimicrobial effects of green synthesized silver nanoparticles using Melissa officinalis grown under in vitro condition

Pirtarighat, Saba and Ghannadnia, Maryam and Baghshahi, Saeid (2017) Antimicrobial effects of green synthesized silver nanoparticles using Melissa officinalis grown under in vitro condition. Nanomedicine Journal, 4 (3). pp. 184-190.

[img]
Preview
Text
NMJ_Volume 4_Issue 3_Pages 184-190.pdf

Download (432kB) | Preview
Official URL: http://nmj.mums.ac.ir/article_8960.html

Abstract

Objective(s): To evaluate the biosynthesis of Ag NPs using plant extract of Melissa officinalis (at the eight leaf stage) grown under in vitro (controlled) condition for the first time.Materials and Methods: Biosynthesis of Ag NPs using plant extract was carried out and formation of Ag NPs confirmed by UV-Visible spectroscopy, X-ray diffraction (XRD), Field Emission Scanning Electron Microscope (FESEM) and Dynamic Light Scattering (DLS). The functional groups of compounds adsorbed on the Ag NPs were identified using Fourier Transform Infrared Spectroscop (FTIR) studies. The antibacterial activity of the Ag NPs was investigated by agar disc diffusion method.Results: The plant extract showed color change in extract from yellow to brown after formation of Ag NPs. The surface Plasmon resonance found at 450 nm confirmed the formation of Ag NPs. FESEM images revealed relatively spherical- shaped of Ag NPs. The biosynthesized Ag NPs were crystalline in nature with mean diameter about 34.64 nm. FTIR results expounded the functional groups of plant extract responsible for the bio-reduction of silver ions and their interaction between them. The obtained nanoparticles showed good inhibitory activity against the Gram positive and Gram negative bacteria.Conclusion: These results suggested that with changes in plants culture condition it may be possible to obtain nanoparticles with desired characteristics.

Item Type: Article
Subjects: QT physiology
Divisions: Journals > Nanomedicine Journal
Depositing User: nmj nmj
Date Deposited: 17 Sep 2017 16:28
Last Modified: 17 Sep 2017 16:28
URI: http://eprints.mums.ac.ir/id/eprint/670

Actions (login required)

View Item View Item