Differentiation of adipocytes and osteocytes from human adipose and placental mesenchymal stem cells

Mohammadi, Zahra and Tavakkol Afshari, Jalil and Keramati, Mohammad Reza and Hamidi Alamdari, Daryoush and Ganjibakhsh, Meysam and Moradi Zarmehri, Azam and Jangjoo, Ali and Sadeghian, Mohammad Hadi and Arab Ameri, Masoumeh and Moinzadeh, Leila (2015) Differentiation of adipocytes and osteocytes from human adipose and placental mesenchymal stem cells. Iranian Journal of Basic Medical Sciences, 18 (3). pp. 259-266.

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Objective(s):Mesenchymal stem cells (MSC) can be isolated from adult tissues such as adipose tissue and other sources. Among these sources, adipose tissue (because of easy access) and placenta (due to its immunomodulatory properties, in addition to other useful properties), have attracted more attention in terms of research. The isolation and comparison of MSC from these two sources provides a proper source for clinical experimentation. The aim of this study was to compare the characteristics of MSC isolated from human adipose tissue and placenta. Materials and Methods: Adipose and placental MSC were isolated from the subcutaneous adipose tissues of 10 healthy women (25 to 40 years) and from a fresh term placenta (n= 1), respectively. Stem cells were characterized and compared by flow cytometry using CD29, CD31, CD34, CD44, CD45, CD105, CD166 and HLA-DR markers. Osteocytes and adipocytes were differentiated from isolated human mesenchymal stem cells (HMSC). Results: Adipose and placenta-derived MSC exhibited the same morphological features. ADSC differentiated faster than placenta; however, both were differentiated, taking up to 21 days for osteocyte and 14 days for adipocyte differentiation. About 90 of PLC-MSC and ADSC were positive for CD29, CD44, CD105, and CD166; and negative for CD31, CD34, CD45, and HLA-DR. Conclusion: The two sources of stem cells showed similar surface markers, morphology and differentiation potential and because of their multipotency for differentiating to adipocytes and osteocytes, they can be applied as attractive sources of MSC for regenerative medicine.

Item Type: Article
Subjects: QT physiology
Divisions: Journals > Iranian J Basic Medical Sciences
Depositing User: ijbms ijbms
Date Deposited: 03 Oct 2017 17:08
Last Modified: 03 Oct 2017 17:08
URI: http://eprints.mums.ac.ir/id/eprint/7404

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