Role of morphine preconditioning and nitric oxide following brain ischemia reperfusion injury in mice

Arabian, Maedeh and Aboutaleb, Nahid and Soleimani, Mansoureh and Mehrjerdi, Fatemeh Zare and Ajami, Marjan and Pazoki-Toroudi, Hamidreza (2015) Role of morphine preconditioning and nitric oxide following brain ischemia reperfusion injury in mice. Iranian Journal of Basic Medical Sciences, 18 (1). pp. 14-21.

IJBMS_Volume 18_Issue 1_Pages 14-21.pdf

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Objective(s): Morphine dependence (MD) potently protects heart against ischemia reperfusion (IR) injury through specific signaling mechanisms, which are different from the pathways involved in acute morphine treatment or classical preconditioning. Since opioid receptor density changes post cerebral ischemia strongly correlated with brain histological damage, in the present study, we tried to elucidate the possible role of opioid receptors in IR injury among morphine-dependent mice. Materials and Methods: Accordingly, incremental doses (10 mg/kg/day to 30 mg/kg/day) of morphine sulphate were subcutaneously administered for 5 days before global brain ischemia induction through bilateral common carotid artery occlusion. Animals were received naloxone (5 mg/kg) or L-NAME (20 mg/kg) 30 min after the last morphine dose. Twenty four hr after the ischemia induction, Retention trial of passive avoidance test and western blot analysis were done. histological analysis (TUNEL and NISSL staining) performed 72 hr after ischemia. Results: MD improved post ischemia memory performance (P<0.01) and neuronal survival (P<0.001) and decreased apoptosis (P<0.05) in region I of hippocampus (CA1F1 M2 region) in mouse. Treatment with naloxone or L-NAME abolished all MD aforementioned effects. Conclusion: Results of the present study suggested that opioid receptors activation in the early hr post ischemia is crucial for MD-induced hippocampus tolerance against IR injury. Opioid receptor-dependent balance of NO production was another key factor in MD-induced protection. Further studies are required to determine the effect of MD on opioid receptor changes after ischemia and its correlation with MD-induced protection.

Item Type: Article
Subjects: QT physiology
QV pharmacology
Divisions: Journals > Iranian J Basic Medical Sciences
Depositing User: ijbms ijbms
Date Deposited: 04 Oct 2017 00:13
Last Modified: 04 Oct 2017 00:13

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