Effect of Nigella sativa on ischemia-reperfusion induced rat kidney damage

Havakhah, Shahrzad and Sadeghnia, Hamid R and Hajzadeh, Mosa-Al-Reza and Mohammadian Roshan, Nama and Shafiee, Somayeh and Hosseinzadeh, Hossein and Mohareri, Narges and Khajavi Rad, Abolfazl (2014) Effect of Nigella sativa on ischemia-reperfusion induced rat kidney damage. Iranian Journal of Basic Medical Sciences, 17 (12). pp. 986-992.

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Abstract

Objective(s):There are a few previously reported studies about the effect of Nigella sativa oil on renal ischemia-reperfusion injury (IRI). The aim of the present study was to test the hypothesis whether pre- or post-treatment with N. sativa hydroalcoholic extract (NSE) would reduce tissue injury and oxidative damages in a clinically relevant rat model of renal IRI. Materials and Methods: IRI was induced by clamping of bilateral renal arteries for 40 min fallowed by reperfusion for 180 min. NSE was prepared in a Soxhlet extractor and administrated with doses of 150 mg/kg or 300 mg/kg at 1 hr before ischemia induction (P-150 and 300) or at the beginning of reperfusion phase (T-150 and 300), via jugular catheter intravenously. The kidneys were then removed and subjected to biochemical analysis, comet assay or histopathological examination. Results: The kidneys of untreated IRI rats had a higher histopathological score (P<0.001), while in P-150, as well as T-150 and T-300 groups tubular lesions significantly decreased (P<0.001). Pre- and post-treatment with NSE also resulted in a significant decrease in malondialdehyde (MDA) level (P<0.001) and DNA damage (P<0.001) that were increased by renal I/R injury. NSE treatment also significantly restore (P<0.01) the decrease in renal thiol content caused by IRI. Conclusion: The present study shows N. sativa extract has marked protective action against renal IRI, which may be partly due to its antioxidant effects.

Item Type: Article
Subjects: QV pharmacology
Divisions: Journals > Iranian J Basic Medical Sciences
Depositing User: ijbms ijbms
Date Deposited: 04 Oct 2017 17:00
Last Modified: 04 Oct 2017 17:00
URI: http://eprints.mums.ac.ir/id/eprint/7539

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