In vitro lethal effects of various extracts of Nigella sativa seed on hydatid cyst protoscoleces

Mahmoudvand, Hossein and Asadi, Arash and Fasihi Harandi, Majid and Sharififar, Fariba and Jahanbakhsh, Sareh and Saedi Dezaki, Ebrahimm (2014) In vitro lethal effects of various extracts of Nigella sativa seed on hydatid cyst protoscoleces. Iranian Journal of Basic Medical Sciences, 17 (12). pp. 1001-1006.

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Abstract

Objective(s): There are several scolicidal agents for inactivation of hydatid cyst protoscolices during surgery, but most of them are associated with adverse side effects such as sclerosing cholangitis and liver necrosis. The present study was aimed to evaluate scolicidal effects of various extracts of Nigella sativa seeds againstprotoscoleces of hydatid cyst in an in vitro model. Materials and Methods: Protoscoleces were aseptically aspirated from naturally infected livers of sheep and goats. Various concentrations of the different extracts of N. sativa (5 to 50 mg/ml) were used for 5 to 60 min. Viability of protoscoleces was confirmed by 0.1 eosin staining. Results: The findings exhibited that methanolic extract at the concentration of 50 mg/ml after 10 min of incubation, and aqueous extract at the concentration of 50 mg/ml after 30 min of incubation can kill 100 of protoscoleces. In addition, all of experiments revealed dose-dependent and also time-dependent scolicidal effect of various extracts of N. sativa on the protoscoleces of hydatid cyst. Conclusion: The results of the present study demonstrated that N. sativa may be a natural source for the production of new scolicidal agent for use in hydatid cyst surgery. However, further studies will be required to evaluate scolicidal effects of N. sativa in the in vivo model.

Item Type: Article
Subjects: QV pharmacology
Divisions: Journals > Iranian J Basic Medical Sciences
Depositing User: ijbms ijbms
Date Deposited: 04 Oct 2017 17:03
Last Modified: 04 Oct 2017 17:03
URI: http://eprints.mums.ac.ir/id/eprint/7541

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