Effect of renal ischemia-reperfusion on lung injury and inflammatory responses in male rat

Yousefi, Hadi and Ahmadiasl, Naser and Alihemmati, Alireza and Habibi, Parisa (2014) Effect of renal ischemia-reperfusion on lung injury and inflammatory responses in male rat. Iranian Journal of Basic Medical Sciences, 17 (10). pp. 802-807.

IJBMS_Volume 17_Issue 10_Pages 802-807.pdf

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Official URL: http://ijbms.mums.ac.ir/article_3457.html


Objective(s):Acute kidney injury (AKI), a syndrome characterized by decreased glomerular filtration, occurs in every 1 of 5 hospitalized patients. Renal ischemia-reperfusion, one of the main causes of AKI, is of particular importance in the setting of kidney transplantation. Materials and Methods: Sixty male rats were divided into four groups including control, nephrectomy, sham surgery and renal ischemia-reperfusion (IRI) group. The rats were anesthetized with intraperitonealketamin and xylazin. For making IRI group, right nephrectomywas performed, and after a week, the left kidney pedicle was occluded for 45 min for making ischemia that followed by 24 hr reperfusion. At the end of reperfusion phase, the lung tissues were isolated to be used in immunohistochemical and histological assays. Immunohistochemical assay was used to evaluate Bcl-2 and TNF-α, and hematoxylin-eosin staining assay was used to histopathology. Results: lung tissues injury after renal ischemia-reperfusion was revealed by immunohistochemistry analysis to increase TNF-α level and decrease Bcl-2 (an anti-apoptotic protein) level. Lung injury and necrosis was discovered by hematoxylin-eosin staining to be more evident in IRI group than sham and control groups. Conclusion: The results demonstrated that increase in TNF-α and decrease in Bcl-2 levels in lungs induces the pulmonary inflammatory damage in renal IRI model.

Item Type: Article
Subjects: QT physiology
QY Clinical Pathology
Divisions: Journals > Iranian J Basic Medical Sciences
Depositing User: ijbms ijbms
Date Deposited: 04 Oct 2017 23:45
Last Modified: 04 Oct 2017 23:45
URI: http://eprints.mums.ac.ir/id/eprint/7606

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