Genetic properties of blaCTX-M and blaPER β-lactamase genes in clinical isolates of Enterobacteriaceae by polymerase chain reaction

Nakhaei Moghaddam, Mahboobeh and Hashemi Beidokhti, Mehrdad and Jamehdar, Saeid Amel and Ghahraman, Martha (2014) Genetic properties of blaCTX-M and blaPER β-lactamase genes in clinical isolates of Enterobacteriaceae by polymerase chain reaction. Iranian Journal of Basic Medical Sciences, 17 (5). pp. 378-383.

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Abstract

Objective(s):blaCTX-Mand blaPER are two genes that encode class A extended-spectrum β-lactamases (ESBLs) and can be responsible for therapeutic problems. This study was carried out to evaluate the molecular properties of these genes in clinical isolates of Enterobacteriaceae by polymerase chain reaction (PCR), restriction digestion and sequencing. Materials and Methods: During six months, starting from January 2012, one hundred clinical isolates of Enterobacteriaceae were collected from urinary samples. The ESBL-producing isolates were detected by phenotypic confirmation test. After plasmid extraction, blaPER and blaCTX-M genes were detected using PCR by specific primers. The blaCTX-M PCR products were digested with Taq1, and two of the blaCTX-Mgenes were sequenced. Results: Phenotypic tests showed that 27 (27) isolates were ESBL producers with the highest frequency for Klebsiella pneumoniae (47.4) andEscherichia coli (17.9). Twenty six (26) of Enterobacteriaceae isolates harbored the blaCTX-Mgene, and none of them had blaPER. The restriction analysis of PCR products showed that all blaCTX-M amplified products had the same patterns. Both sequenced bacteria were CTX-M-15 type ESBL carriers. Conclusion: The results of this study showed the blaCTX-M-15gene in Enterobacteriaceae isolates for the first time in Mashhad, Iran. High degrees of associated resistance to co-trimoxazole and gentamicin were found in ESBL producers. Therefore, an integrated and regular management of antibiotic prescription need to be trained in our society.

Item Type: Article
Subjects: QW Microbiology and Immunology
Divisions: Journals > Iranian J Basic Medical Sciences
Depositing User: ijbms ijbms
Date Deposited: 07 Oct 2017 18:29
Last Modified: 07 Oct 2017 18:29
URI: http://eprints.mums.ac.ir/id/eprint/7915

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