Identification of Novel Hypoxia Response Genes in Human Glioma Cell Line A172

Baghbani, Fatemeh and Raoofian, Reza and Hasanzadeh Nazarabadi, Mohammad and Hamzehloei, Tayebeh and Soukhtanloo, Mohammad and Heidari, Mansur and Afsharzadeh, Seyed Morteza and Shekouhi, Sahar and Moradi, Fahimeh and Sarli, Abdol-Azim and Zavar-Reza, Javad and Mojarrad, Majid (2013) Identification of Novel Hypoxia Response Genes in Human Glioma Cell Line A172. Iranian Journal of Basic Medical Sciences, 16 (5). pp. 675-682.

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Objective(s): Hypoxia is a serious challenge for treatment of solid tumors. This condition has been manifested to exert significant therapeutic effects on glioblastoma multiform or (WHO) astrocytoma grade IV. Hypoxia contributes numerous changes in cellular mechanisms such as angiogenesis, metastasis and apoptosis evasion. Furthermore, in molecular level, hypoxia can cause induction of DNA breaks in tumor cells. Identification of mechanisms responsible for these effects can lead to designing more efficient therapeutic strategies against tumor progression which results in improvement of patient prognosis. Materials and Methods: In order to identify more hypoxia regulated genes which may have a role in glioblastoma progression, cDNA-AFLP was optimized as a Differential display method which is able to identify and isolate transcripts with no prior sequence knowledge. Results: Using this method, the current study identified 120 Transcription Derived Fragments (TDFs) which were completely differentially regulated in response to hypoxia. By sequence homology searching, the current study could detect 22 completely differentially regulated known genes and two unknown sequence matching with two chromosome contig and four sequence matches with some Expressed Sequence Tags (ESTs). Conclusion: Further characterizing of these genes may help to achieve better understanding of hypoxia mediated phenotype change in tumor cells.

Item Type: Article
Subjects: QT physiology
Divisions: Journals > Iranian J Basic Medical Sciences
Depositing User: ijbms ijbms
Date Deposited: 18 Oct 2017 15:25
Last Modified: 18 Oct 2017 15:25

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