Induction of Apoptosis in the Rat Bone Marrow Mesenchymal Stem Cells Following Sodium Arsenite Treatment with the Dose Lesser than that Used for Treatment of Malignant Patient

Abnosi, Mohammad Husein and Mehranjani1, Malek Solemani and Momeni, Hamidreza and Shojafar, Elham and Barati, Mozhgan (2012) Induction of Apoptosis in the Rat Bone Marrow Mesenchymal Stem Cells Following Sodium Arsenite Treatment with the Dose Lesser than that Used for Treatment of Malignant Patient. Iranian Journal of Basic Medical Sciences, 15 (4). pp. 900-906.

[img]
Preview
Text
IJBMS_Volume 15_Issue 4_Pages 900-906.pdf

Download (158kB) | Preview
Official URL: http://ijbms.mums.ac.ir/article_4877.html

Abstract

Objective(s) Arsenic compounds are potent human carcinogen and produce a variety of stress responses in mammalian cells. Recently sodium arsenite has been recommended to be used as anti malignancy drug by American food and drug administration (FDA). In this study, we aimed to determine the apoptosis inducing effect of sodium arsenite on rat bone marrow mesenchymal stem cells exposed in vitro. Methodology Cell morphology was studied with the help of Hoechst and propidium iodide as well as with single cell gel electrophoresis (comet assay), TUNEL assay and caspase activity base on immunocytochemistry using commercial kit were considered to study the mechanism of cell death. Results Our result showed that the sodium arsenite with concentration of 0.1 µM in 36 hr induces caspase dependent apoptosis in rat bone marrow mesenchymal stem cells. This concentration is the lowest level of sodium arsenite to be reported with apoptosis induction ability in stem cells. Conclusion Since sodium arsenite is used in therapy, more research should be carried out on the effect of this chemical on stem cells, especially MSCs.

Item Type: Article
Subjects: QV pharmacology
Divisions: Journals > Iranian J Basic Medical Sciences
Depositing User: ijbms ijbms
Date Deposited: 28 Oct 2017 15:49
Last Modified: 28 Oct 2017 15:49
URI: http://eprints.mums.ac.ir/id/eprint/8382

Actions (login required)

View Item View Item