The Mucosal Adjuvant Potential of Cross-Linked Dextran Microspheres as Dry Powder

Tafaghodi, Mohsen and Eskandari, Maryam (2012) The Mucosal Adjuvant Potential of Cross-Linked Dextran Microspheres as Dry Powder. Iranian Journal of Basic Medical Sciences, 15 (3). pp. 873-879.

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Abstract

Objective(s) The immunoadjuvant potential of cross-linked dextran microspheres (CDM) as absorption enhancer and Quillaja saponins (QS) as immunomodulator adjuvant was evaluated. Materials and Methods CDM loaded or tetanus-mixed toxoid (TT) or Quillaja saponin (QS) were nasally administered to rabbits in dry powder form, three times in 2 weeks interval and serum IgG and nasal lavage sIgA titers were determined by ELISA. Results The highest serum IgG titer was induced by parenteral immunization through alum adsorbed TT (P< 0.001). Among nasally immunized groups, the highest serum IgG titer was induced by (TT+QS)CDM (P< 0.01). Mixing of CDM with TT+QS powders (CDM+TT+QS), could not induce the high serum IgG titers as (TT+QS)CDM (P< 0.01). CDM loaded with TT+QS induced higher IgG titers than CDM loaded with TT alone (P< 0.01). No significant difference was observed in nasal lavage sIgA titers of various groups. Conclusion CDM microspheres loaded with TT+QS significantly increased serum anti-TT IgG titers, but mixing of CDM with TT+QS powder could not increase IgG titers. Both QS and CDM adjuvant could not significantly increase the lavage anti-TT IgA titers.

Item Type: Article
Subjects: QV pharmacology
Divisions: Journals > Iranian J Basic Medical Sciences
Depositing User: ijbms ijbms
Date Deposited: 29 Oct 2017 15:55
Last Modified: 29 Oct 2017 15:55
URI: http://eprints.mums.ac.ir/id/eprint/8440

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