Identification and Characterization of a High Vancomycin-Resistant Staphylococcus aureus Harboring VanA Gene Cluster Isolated from Diabetic Foot Ulcer

Dezfulian, Anahita and Aslani, Mohammad Mehdi and Oskoui, Mahvash and Farrokh, Parisa and Azimirad, Masumeh and Dabiri, Hossein and Salehian, 1Mohammad Taghi and Zali, 1Mohammad Reza (2012) Identification and Characterization of a High Vancomycin-Resistant Staphylococcus aureus Harboring VanA Gene Cluster Isolated from Diabetic Foot Ulcer. Iranian Journal of Basic Medical Sciences, 15 (2). pp. 783-786.

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Abstract

Objective(s) Staphylococcus aureus is a common cause of human infection, and emergence of vancomycin-resistance S. aureus is a great concern for treatment of methicillin-resistant S. aureus,(MRSA) in recent years (MRSA). Here, we report the isolation of high-level VRSA. Materials and Methods S. aureus was isolated from foot ulcer of a diabetic woman in Tehran, Iran. Antibiotic susceptibility was determined according to CLSI guidelines. VanA gene cluster PCR was carried out and PCR amplicon of vanA was sequenced. Results S. aureus had high-level vancomycin-resistant (MIC 512 ≥ µg/ml). Patient's history revealed that VRSA isolate was acquired through community transmission. Only vanA, vanR and vanS genes were amplified in our isolate. Sequencing revealed that the vanA sequence had high similarity to the vanA sequence of Tn1546. Conclusion Although VRSA infection continues to be rare, isolation of community–acquired VRSA is a significant issue and it needs the efforts of public health authorities.

Item Type: Article
Subjects: QW Microbiology and Immunology
Divisions: Journals > Iranian J Basic Medical Sciences
Depositing User: ijbms ijbms
Date Deposited: 31 Oct 2017 17:13
Last Modified: 31 Oct 2017 17:13
URI: http://eprints.mums.ac.ir/id/eprint/8455

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