The role of TSG-6 and uroplakin III in bladder pain syndrome/ interstitial cystitis in rats and humans

Lv, Yi-Song and Gao, Rui and Lin, Qing-Ming and Jiang, Tao and Chen, Qin and Tang, Song-Xi and Mao, Hou-Ping and Zhou, Hui-Liang and Cao, Lin-Sheng (2017) The role of TSG-6 and uroplakin III in bladder pain syndrome/ interstitial cystitis in rats and humans. Iranian Journal of Basic Medical Sciences, 20 (11). pp. 1242-1249.

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Abstract

Objective(s):We investigated the relationship between the expression of tumor necrosis factor-inducible gene 6 (TSG-6) with inflammation and integrity of the bladder epithelium in the bladder tissues of patients with bladder pain syndrome/interstitial cystitis (BPS/IC) and the mechanism of action using a rat model of BPS/IC. Materials and Methods: Expression of TSG-6 and uroplakin III was determined by immuno- histochemistry of bladder biopsy samples from control human subjects and patients with verified BPS/IC. Our rat model of BPS/IC was employed to measure the perfusion of bladders with hyaluronidase, and assessment of the effect of TSG-6 administration on disease progression. Treatment effects were assessed by measurement of metabolic characteristics, RT-PCR of TGR-6 and interleukin-6, bladder histomorphology, and immunohistochemistry of TGR-6 and uroplakin III. Results: The bladders of patients with BPS/IC had lower expression of uroplakin III and higher expression of TSG-6 than controls. Rats treated with hyaluronidase for 1 week developed the typical signs and symptoms of BPS/IC, and rats treated with hyaluronidase for 4 weeks had more serious disease. Administration of TSG-6 reversed the effects of hyaluronidase and protected against disease progression. Conclusion:Our results indicate that TSG-6 plays an important role in maintaining the integrity of the bladder epithelial barrier.

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