Increased risk of gastric cancer with decreased numbers of VNTR in the promoter region of RhoB gene and assessment of repeats number effect on expression of RhoB gene

shakeri, fariba and Mohammadinejad, parisa and Moghanibashi, Mohammad Mahdi (2017) Increased risk of gastric cancer with decreased numbers of VNTR in the promoter region of RhoB gene and assessment of repeats number effect on expression of RhoB gene. medical journal of mashhad university of medical sciences, 59 (6). pp. 293-304.

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Abstract

Abstract Introduction: The RhoB gene is one of the genes that are involved in cancers such as gastric cancer. This gene is contributed in cytoskeletal organization and cell trafficking and its expression is decreased in gastric cancer. There are a VNTR polymorphism in the promoter of the RhoB gene and in this study different genotypes of this VNTR was assessed with risk of gastric cancer, for the first time. Materials and Methods: In this study, the VNTR polymorphism of the promoter region in 250 controls and 100 patients with gastric cancer, was assessed by PCR and electrophoresis on agarose gel. Then, the result was assessed by regression logistic and X2 tests. Also expression of the RhoB gene in 19 controls with different repeats in promoter region was compared by Real Time RT-PCR. Results: Our result showed the 1.9 folds increase risk of gastric cancer with 8 and 9 repeats allele in comparison with 10 and 11 repeats (p=0.044 ). But we found no association of number of repeats with expression of RhoB gene (p=0.605). Conclusion:The VNTR polymorphism of the RhoB genepromoter is associated with risk of gastric cancer and may be used for screening of gastric cancer if was confirmed by other studies.

Item Type: Article
Subjects: WA Public Health
Divisions: Journals > Medical J Mashhad University of Medical Sciences
Depositing User: mjmums mjmums
Date Deposited: 26 Nov 2017 17:30
Last Modified: 26 Nov 2017 17:30
URI: http://eprints.mums.ac.ir/id/eprint/8617

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