Effects of flavonoids from Martynia annua and Tephrosia purpurea on cutaneous wound healing

Lodhi, Santram and Jain, Avijeet and Jain, Alok Pal and Pawar, Rajesh and Singhai, Abhay Kumar (2016) Effects of flavonoids from Martynia annua and Tephrosia purpurea on cutaneous wound healing. Avicenna Journal of Phytomedicine, 6 (5). pp. 578-591.

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Abstract

Objective: Martynia annua L. (M. annua), (Martyniaccae) has been traditionally used in the treatment of epilepsy, sore throat and inflammatory disorders. The leaf paste is used topically on Tuberculosis of the lymphatic glands and wounds of domestic animals. Tephrosia purpurea (T. purpurea), (Fabaceae) has been used traditionally as a remedy for asthma, gonorrhea, rheumatism and ulcers. This study aimed to evaluate the potential wound healing effects of different fractions ofethanol extract of M. annua leaves and aerial parts of T. purpurea. Materials and Methods: Methanol fraction of M. annua (MAF-C) and ethyl acetate fraction of T. purpurea (TPF-A) were evaluated for healing potential in dead-space and burn wound models. An ointment (5 w/w) of MAF-C and TPF-A, pongamol (0.2 and 0.5 w/w) and luteolin (0.2 and 0.5 w/w) was applied topically twice a day. The effects were compared with Povidone Iodine ointment with respect to protein, collagen content, enzymatic assay and histopathological finding of granuloma tissues. Results: Ethanol extracts of M. annua and T. purpureawere exhibited total flavonoid contents of 126.2 ± 4.69 and 171.6 ± 6.38 mg (quercetin equivalent), respectively. HPLC fingerprinting confirmed the presence of luteolin in M. annua and quercetin in T. purpurea. TPF-A and MAF-C ointments (5 w/w) significantly increases the hydroxyproline and protein contents. Luteolin and pongamol ointments were also found to be effective in both wound models. Conclusion: Our findings suggested that 5 w/w ointment of TPF-A and MAF-C fractions were more effective than isolated flavonoids in wound healing which may be due to synergistic interactions between the flavonoids and other constituents.

Item Type: Article
Subjects: WR Dermatology
Divisions: Journals > Avicenna J Phytomedicine
Depositing User: ajp ajp
Date Deposited: 18 Sep 2017 16:09
Last Modified: 18 Sep 2017 16:09
URI: http://eprints.mums.ac.ir/id/eprint/892

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