Genetically Engineered Mesenchymal Stem Cells Stably Expressing Green Fluorescent Protein

Halabian, Raheleh and Mohammadi, Mohamad Hosein and Salimi, Mohammad and Amani, Maryam and Mohammadi Roushande, Amaneh and Aghaipoor, Mahnaz and Amirizadeh, Nasser and Ebrahimi, Majid and Jahanian Najafabadi, Ali and Habibi Roudkenar, Mehryar (2010) Genetically Engineered Mesenchymal Stem Cells Stably Expressing Green Fluorescent Protein. Iranian Journal of Basic Medical Sciences, 13 (2). pp. 24-30.

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Abstract

Objective(s) Mesenchymal stem cells (MSCs) are nonhematopoietic stromal cells that are capable of differentiating into and contribute to the regeneration of mesenchymal tissues. Human mesenchymal stem cells (hMSCs) are ideal targets in cell transplantation and tissue engineering. Enhanced green fluorescent protein (EGFP) has been an important reporter gene for gene therapy. The aim of this study was establishment of MSCs expressing GFP. Materials and Methods MSCs were isolated and characterized by Immunophenotyping. The pEGFP-N1 plasmid was extracted from previously transformed Escherichia. coli cells and transfected into MSCs using FuGENE HD transfection reagent. Stable cells were established in the presence of geneticin. Expression of GFP was detected by RT- PCR, western blot analysis and immunoflorecent microscope. Results MSCs were successfully isolated and characterized. The MSCs transfected with the pEGFP-N1 plasmid expressed GFP both in mRNA and protein levels while cells transfected with empty vector did not. Conclusion The results suggested that this engineered cell line will be used in the future studies and can easily be traced in vivo.

Item Type: Article
Subjects: QV pharmacology
Divisions: Journals > Iranian J Basic Medical Sciences
Depositing User: ijbms ijbms
Date Deposited: 07 Feb 2018 16:00
Last Modified: 07 Feb 2018 16:00
URI: http://eprints.mums.ac.ir/id/eprint/9197

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