Evaluation of the Effects of the Concentration of Ozonated Water on the Inhibition of Candida albicans Colonization and Plaque Formation on Acrylic Dental Plates

Ariamanesh, Hafez and Malimir, Fatemeh and Babaei, Maryam and Motamed, Nima and Amanloo, Saeid (2018) Evaluation of the Effects of the Concentration of Ozonated Water on the Inhibition of Candida albicans Colonization and Plaque Formation on Acrylic Dental Plates. Journal of Mashhad Dental School, 42 (2). pp. 95-104.

[img] Text
JMDS_Volume 42_Issue 2_Pages 95-104.pdf

Download (558kB)
Official URL: http://jmds.mums.ac.ir/article_10884.html

Abstract

Introduction: The formation of dental plaques is one of the underlying causes of denture-related stomatitis. This study was conducted to evaluate the effect of the concentration of ozonated water on the inhibition of the formation of Candida albicans plaque on the acrylic resin pieces. Materials and Methods: In this study, 45 resin acrylic plates were contaminated by Candida albicans suspension, which were randomly divided into nine groups. They were treated with 0.2, 0.5, 1, and 2 µg/ml of ozonated water, 25 and 12.5 µg/ml of ozonized oil, 100,000 units of nystatin (positive control), distilled water, and olive oil (negative control). Thereafter, the solution obtained from rinsing the pieces was cultured in Sabouraud Dextrose Agar and the mean number of the colonies was Mann-Whitney U and kruskal-wallis tests were for statistical analysis. Results: The mean number of the colonies obtained at the concentrations of 0.2, 0.5, 1, and 2 μg/ml of ozonated water was 24, 24.6, 23.6, and 14.4 colonies, respectively. Additionally, the mean number of the colonies obtained at the concentrations of 12.5 and 25 μg/ml of ozonized oil was 0 and 2 colonies, respectively. There was a significant difference between the solutions cultured in distilled water (146.6) and olive oil (98.8) in terms of the number of colonies (P<0.001) . In all the groups, the number of the yeast colonies decreased by increasing the concentration of ozone. However, ozonized oil had more inhibitory effect than ozonated water. There was no significant difference between the solutions cultured in two concentrations of ozonized oil (25 µg/ml P=1 and 12.5 µg/ml P=0.477) and nystatin. Conclusion: Appropriate concentrations of ozonated water had an antifungal effect on Candida albicans. Therefore, further clinical studies are recommended to standardize and elaborate the guidelines for using ozone therapy.

Item Type: Article
Subjects: WU Dentistry. Oral surgery
Divisions: Journals > Journal of Mashhad Dental School
Depositing User: jmds jmds
Date Deposited: 14 Jun 2018 06:38
Last Modified: 14 Jun 2018 06:38
URI: http://eprints.mums.ac.ir/id/eprint/9793

Actions (login required)

View Item View Item