An answer to colon cancer treatment by mesenchymal stem cell originated from adipose tissue

Iplik, Elif and Ertugrul, Baris and Kozanoglu, İlknur and Baran, Yusuf and Cakmakoglu, Bedia (2018) An answer to colon cancer treatment by mesenchymal stem cell originated from adipose tissue. Iranian Journal of Basic Medical Sciences, 21 (5). pp. 465-468.

[img] Text
IJBMS_Volume 21_Issue 5_Pages 465-468.pdf

Download (434kB)
Official URL: http://ijbms.mums.ac.ir/article_10541.html

Abstract

Objective(s): Colon cancer is risen up with its complex mechanism that directly impacts on its treatment as well as its common prevalence. Mesenchymal stem cells (MSCs) have been considered as a therapeutic candidate for conventional disease including cancer. In this research, we have focused on apoptotic effects of adipose tissue-derived MSCs in colon cancer.Materials and Methods: MSCs were obtained from adipose tissue and characterized by Flowcytometer using suitable antibodies. MSCs, HT-29, HCT-116, RKO and healthy cell line MRC5 were cultured by different seeding procedure. After cell viability assay, changes in caspase 3 enzyme activity and the level of phosphatidylserine were measured.Results: For cell viability assay, a 48 hr incubation period was chosen to seed all cells together. There was a 1.36-fold decrease in caspase 3 enzyme activity by co-treatment of RKO and MSCs in addition to 2.02-fold decrease in HT-29 and MSCs co-treatment, and 1.103-fold increase in HCT-116 and MSCs. The results demonstrated that HCT-116 led to the highest rate of apoptotic cell death (7.5) compared with other cells.Conclusion: We suggest that MSCs might remain a new treatment option for cancer by its differentiation and repair capacity.

Item Type: Article
Subjects: QV pharmacology
Divisions: Journals > Iranian J Basic Medical Sciences
Depositing User: ijbms ijbms
Date Deposited: 25 Jul 2018 04:55
Last Modified: 25 Jul 2018 04:55
URI: http://eprints.mums.ac.ir/id/eprint/9949

Actions (login required)

View Item View Item